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Multicenter study of automated systems for colistin susceptibility testing
European Journal of Clinical Microbiology & Infectious Diseases ( IF 4.5 ) Pub Date : 2020-10-06 , DOI: 10.1007/s10096-020-04059-4
Ahalieyah Anantharajah 1 , Youri Glupczynski 1 , Martin Hoebeke 1 , Pierre Bogaerts 1 , Philippe Declercq 2 , Olivier Denis 1, 3, 4 , Julie Descy 5 , Katelijne Floré 6 , Koen Magerman 7 , Hector Rodriguez-Villalobos 8 , Anne-Marie Van den Abeele 9 , Te-Din Huang 1
Affiliation  

Purpose

Broth microdilution (BMD) stays as the reference testing method for determination of antimicrobial susceptibility testing (AST) to colistin and is considered essential for patient management and for monitoring of colistin resistance. This multicenter study aimed to evaluate the performance of automated systems for colistin AST among Enterobacterales as an alternative for BMD since the majority of laboratories use automated systems as first-line method.

Methods

Twenty colistin resistant (COL-R) including 10 MCR producers and 10 colistin-susceptible (COL-S) Enterobacterales isolates were blindly tested for colistin susceptibility with the routine automated AST systems used by 8 laboratories (3 with BD Phoenix, 3 with Vitek2 and 2 with MicroScan). Additionally, 3 reference strains (E. coli ATCC 25922, E. coli NCTC 13846, and one COL-R mcr-negative K. pneumoniae M/14750) were tested in triplicate by each laboratory.

Results and conclusion

Results were compared with BMD performed at the reference laboratory. BD Phoenix and MicroScan automated AST systems provide accurate and reproducible categorical results for the testing of colistin in Enterobacterales. However, Vitek2 system showed poor performance for the detection of COL-R isolates especially those with MICs close to the susceptibility breakpoint (categorical agreement of 88% and precision categorical agreement of 81%).



中文翻译:

粘菌素药敏试验自动化系统的多中心研究

目的

肉汤微量稀释 (BMD) 仍然是确定对粘菌素的抗微生物药敏试验 (AST) 的参考测试方法,被认为对患者管理和监测粘菌素耐药性至关重要。这项多中心研究旨在评估肠杆菌中粘菌素 AST 自动化系统作为 BMD 替代方案的性能,因为大多数实验室使用自动化系统作为一线方法。

方法

20 种粘菌素耐药 (COL-R),包括 10 种 MCR 生产者和 10 种粘菌素敏感 (COL-S) 肠杆菌分离株,使用 8 个实验室(3 个使用 BD Phoenix,3 个使用 Vitek2 和2 与 MicroScan)。此外,每个实验室对3 种参考菌株(大肠杆菌ATCC 25922、大肠杆菌NCTC 13846 和一种 COL-R mcr 阴性肺炎克雷伯菌M/14750)进行了三次重复测试。

结果与结论

结果与在参考实验室进行的 BMD 进行了比较。BD Phoenix 和 MicroScan 自动化 AST 系统可为肠杆菌中粘菌素的检测提供准确且可重复的分类结果。然而,Vitek2 系统在检测 COL-R 分离株方面表现不佳,尤其是那些 MIC 接近易感性断点的分离株(分类一致性为 88%,精确分类一致性为 81%)。

更新日期:2020-10-07
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