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Fatal neonatal infection with Klebsiella pneumoniae in dromedary camels: pathology and molecular identification of isolates from four cases
Tropical Animal Health and Production ( IF 1.7 ) Pub Date : 2020-10-06 , DOI: 10.1007/s11250-020-02421-4
Shirish Dadarao Narnaware , Shyam Singh Dahiya , Rakesh Ranjan , Fateh Chand Tuteja

In the present study, sudden mortalities were reported due to pneumonia in four neonatal camels (5 to 10 days old) of an organized dromedary camel farm. The clinical manifestations in affected camels were weakness, mild to high fever, not suckling, respiratory distress, and sudden death. On the basis of gross and histopathological lesions, the pneumonia was classified into bronchopneumonia (n = 2), bronchointerstitial pneumonia (n = 1), and interstitial pneumonia (n = 1). In bacterial culture, Klebsiella pneumoniae was isolated from lung of all the four cases. These bacteria were also confirmed by PCR from DNA isolated from culture as well as lung tissue. The sequencing of PCR products from four isolates of K. pneumoniae revealed homology with other pathogenic K. pneumoniae isolates from India and other countries. These findings confirmed the role of K. pneumoniae as an important etiological agent for causing pneumonia with sudden mortalities in suckling neonatal dromedary camels.



中文翻译:

单峰骆驼致命性新生儿肺炎克雷伯菌感染:4例分离株的病理学和分子鉴定

在本研究中,据报道,在一个有组织的单峰骆驼农场的四只新生骆驼(5至10天大)中,由于肺炎导致猝死。受影响的骆驼的临床表现为虚弱,轻度至高烧,不哺乳,呼吸窘迫和猝死。毛上和组织病理学病变的基础上,肺炎被分类为支气管肺炎(Ñ  = 2),bronchointerstitial肺炎(Ñ  = 1),和间质性肺炎(Ñ  = 1)。在细菌培养中,从四例病例的肺中分离出肺炎克雷伯菌。这些细菌也通过PCR从培养物和肺组织分离的DNA中得到证实。四种肺炎克雷伯菌分离株PCR产物的测序与印度和其他国家的其他致病性肺炎克雷伯菌分离株具有同源性。这些发现证实了肺炎克雷伯菌(K.pneumoniae)作为重要的病原体在哺乳新生儿单峰骆驼中引起猝死性肺炎的重要作用。

更新日期:2020-10-07
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