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Prevalence of R Genes for Resistance to Potato Viruses in Uganda Germplasm
Potato Research ( IF 2.9 ) Pub Date : 2020-10-06 , DOI: 10.1007/s11540-020-09477-7
Arinaitwe Abel Byarugaba , Settumba B. Mukasa , Alex Barekye , Patrick R. Rubaihayo

The most important potato viruses in Uganda are Potato virus Y (PVY), Potato leafroll virus (PLRV), Potato virus X (PVX), Potato virus S (PVS), Potato virus A (PVA) and Potato virus M (PVM). Utilization of R genes in breeding for resistance to viruses has not been explored in Uganda due to limited information on the prevalence of R genes in the available genotypes. This study aimed at identifying potato genotypes with R genes for resistance to potato viruses important in Uganda. The study screened 71 potato accessions from the National Potato Breeding Programme at Kachwekano Zonal Agricultural Research and Development Institute for the presence of resistance genes to viruses using diagnostic molecular markers. The results indicated that 21 out of 71 genotypes had resistance markers, of which nine genotypes, NKRN59.58, Derby, Markies, Sifra, 395017.229, Nakpot 5, 20108.5, Royal and 393220.54, had Ryadg gene for PVY resistance and two genotypes, Kimuri and 319919.3, had Rysto gene for resistance to PVY and PVA. Nine genotypes, 395011.2, Markies, Nakpot 5, 20108.5, Sifra, 20157.6, Royal, 2015.8 and Ambition, had the Nbtbr gene for resistance to PVX. In addition, 14 genotypes, 395011.2, Markies, Nakpot 5, Sarpouna, 393220.54, 391046.14, Sarpomira, 395077.12, Sifra, 20157.6, Royal, Ambition, Kimuri and Caruso, had the Nsadg gene conferring resistance to PVS. Four genotypes, Markies, Sifra, Nakpot 5 and Royal, had the Ryadg, Nbtbr and Nsadg genes for combined resistance to PVY, PVX and PVS. The resistant genotypes could be used as parents to introgress resistance genes into susceptible cultivars.



中文翻译:

乌干达种质对马铃薯病毒抗性的R基因的流行

乌干达最重要的马铃薯病毒是马铃薯病毒Y(PVY),马铃薯卷叶病毒(PLRV),马铃薯病毒X(PVX),马铃薯病毒S(PVS),马铃薯病毒A(PVA)和马铃薯病毒M(PVM)。由于有关可用基因型中R基因普遍性的信息有限,在乌干达尚未探索将R基因用于抗病毒育种。这项研究旨在鉴定具有R的马铃薯基因型对乌干达很重要的马铃薯病毒抗性基因。这项研究使用诊断分子标记从卡奇维卡诺地区农业研究与发展研究所的国家马铃薯育种计划中筛选了71种马铃薯种质,以寻找对病毒的抗性基因。结果表明,在71个基因型中有21个具有抗性标记,其中NKRN59.58,Derby,Markies,Sifra,395017.229,Nakpot 5、20108.5,Royal和393220.54这9个基因型具有PVY抗性的Ry adg基因和两个基因型。 Kimuri和319919.3具有Ry sto基因,可抵抗PVY和PVA。9种基因型395011.2,Markies,Nakpot 5、20108.5,Sifra,20157.6,Royal,2015.8和Ambition具有Nb tbr对PVX有抗性的基因。另外,14个基因型395011.2,Markies,Nakpot 5,Sarpouna,393220.54、391046.14,Sarpomira,395077.12,Sifra,20157.6,Royal,Ambition,Kimuri和Caruso具有Ns adg基因赋予对PVS的抗性。四种基因型,Markies,Sifra,Nakpot 5和Royal具有Ry adgNb tbrNs adg基因,对PVY,PVX和PVS具有综合抗性。抗性基因型可用作亲本,将抗性基因渗入易感品种。

更新日期:2020-10-07
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