The application of plant suspension culture to produce valuable compounds, such as the triterpenoids oleanolic acid and ursolic acid, is a well-established alternative to the cultivation of whole plants. Cambial meristematic cells (CMCs) are a growing field of research, often showing superior cultivation properties compared to their dedifferentiated cell (DDC) counterparts. In this work, the first-time establishment of O. basilicum CMCs is demonstrated. DDCs and CMCs were cultivated in shake flasks and wave-mixed disposable bioreactors (wDBRs) and evaluated regarding triterpenoid productivity and biomass accumulation. CMCs showed characteristic small vacuoles and were found to be significantly smaller than DDCs. Productivities of oleanolic and ursolic acid of CMCs were determined at 3.02 ± 0.76 mg/(l*d) and 4.79 ± 0.48 mg/(l*d) after 19 days wDBR cultivation, respectively. These values were consistently higher than any productivities determined for DDCs over the observed cultivation period of 37 days. Elicitation with methyl jasmonate of DDCs and CMCs in shake flasks resulted in increased product contents up to 48 h after elicitor addition, with the highest increase found in CMCs at 232.30 ± 19.33% (oleanolic acid) and 192.44 ± 18.23% (ursolic acid) after 48 h.

应用植物悬浮培养产生有价值的化合物，例如三萜类齐墩果酸和熊果酸，是栽培整株植物的公认方法。冈比亚分生细胞（CMC）是一个正在发展的研究领域，与脱分化细胞（DDC）相比，它们通常显示出优越的培养特性。在这项工作中，第一次建立了O. basilicum演示了CMC。DDC和CMC在摇瓶和波状混合的一次性生物反应器（wDBR）中进行培养，并评估三萜类化合物的生产率和生物量积累。CMC显示出特征性的小液泡，并且被发现比DDC小得多。wDBR培养19天后，CMC的齐墩果酸和熊果酸的生产率分别确定为3.02±0.76 mg /（l * d）和4.79±0.48 mg /（l * d）。这些值始终高于在观察到的37天培养期内为DDC确定的任何生产率。加入摇瓶后，在摇瓶中用茉莉酸甲酯DDC和CMC进行培养，导致产品含量增加，在CMC中最高，分别为232.30±19.33％（油酸）和192.44±18.23％（熊果酸）。 48小时