Background

Acinetobacter baumannii is a major opportunistic pathogen causing nosocomial infections. Acinetobacter baumannii possesses a quorum sensing system consisting of abaI, encoding an autoinducer synthase, and abaR, encoding a putative LuxR type regulator. AbaI is required for motility and biofilm formation in A. baumannii. However, the functions of AbaR on the expression of abaI, motility, and the formation of biofilm and pellicle have not yet been explored.

Objective

The aim of this study was to investigate the effects of abaR mutation on the expression of abaI, motility, and the formation of biofilm and pellicle.

Methods

Functions of AbaR were assessed by the construction of an isogenic mutant and by evaluating the effects of abaR mutation on the expression of abaI, motility, and the formation of biofilm and pellicle.

Results

The abaR mutant revealed a significant decrease in the expression of abaI. The disruption of abaR resulted in substantial defects in motility and the formation of biofilm and pellicle. Introduction of abaR in trans complemented the defects.

Conclusions

AbaR of A. baumannii is required for the expression of abaI and plays important roles in motility and the formation of biofilm and pellicle. AbaR may be considered to be a target of anti-biofilm agents.

中文翻译：

---

AbaR 是一种 LuxR 型调节剂，对于鲍曼不动杆菌中的运动和生物膜和薄膜的形成至关重要

背景

鲍曼不动杆菌是引起医院感染的主要机会性病原体。鲍曼不动杆菌拥有一个群体感应系统，由abaI和abaR组成，abaI编码自诱导剂合酶，abaR编码推定的 LuxR 型调节器。AbaI 是鲍曼不动杆菌运动和生物膜形成所必需的。然而，ABAR对表达的功能ABAI，运动性，以及生物膜和薄膜的形成还没有被开发。

客观的

本研究的目的是研究abaR突变对abaI表达、运动性以及生物膜和薄膜形成的影响。

方法

AbaR 的功能通过构建等基因突变体和评估abaR突变对abaI表达、运动性以及生物膜和薄膜形成的影响来评估。

结果

所述ABAR突变体揭示的表达的减少显著ABAI。abaR的破坏导致运动性和生物膜和薄膜形成的实质性缺陷。反式引入abaR弥补了这些缺陷。

结论

A 的AbaR 。鲍曼不动杆菌是abaI表达所必需的，并且在运动和生物膜和薄膜的形成中起着重要作用。AbaR 可能被认为是抗生物膜剂的目标。