The perineurium serves as a selective, metabolically active diffusion barrier in the peripheral nervous system, which is composed of perineurial cells joined together by tight junctions (TJs). Not only are these junctions known to play an essential role in maintaining cellular polarity and tissue integrity, but also limit the paracellular diffusion of certain molecules and ions, whereas loss of TJs barrier function is imperative for tumour growth, invasion and metastasis. Hence, a detailed study on the barrier function of perineurial cells may provide insights into the molecular mechanism of perineural invasion (PNI). In this study, we aimed to develop an efficient procedure for the establishment of perineurial cell lines as a tool for investigating the physiology and pathophysiology of the peripheral nerve barriers. Herein, the isolation, expansion, characterization and maintenance of perineurial cell lines under favourable conditions are presented. Furthermore, the analysis of the phenotypic features of these perineurial cells as well as the barrier function for the study of PNI are described. Such techniques may provide a valuable means for the functional and molecular investigation of perineurial cells, and in particular may elucidate the pathogenesis and progression of PNI, and other peripheral nerve disorders.

PERINEURIUM用作周围神经系统中的选择性代谢活性扩散屏障，其由通过紧密结（TJ）连接在一起的睫毛细胞组成。已知这些连接不仅在维持细胞极性和组织完整性方面发挥重要作用，而且还限制某些分子和离子的细胞旁扩散，而 TJ 屏障功能的丧失对于肿瘤生长、侵袭和转移是必不可少的。因此，对神经周围细胞屏障功能的详细研究可能有助于深入了解神经周围侵袭（PNI）的分子机制。在这项研究中，我们旨在开发一种有效的程序来建立神经周围细胞系，作为研究周围神经屏障的生理学和病理生理学的工具。在这里，隔离，介绍了在有利条件下神经周围细胞系的扩增、表征和维持。此外, 还描述了对这些神经周围细胞的表型特征以及 PNI 研究的屏障功能的分析。这种技术可以为神经周围细胞的功能和分子研究提供有价值的手段，特别是可以阐明 PNI 和其他周围神经疾病的发病机制和进展。