Decellularization is a promising method for obtaining extracellular matrix scaffolds (ECM) to be used as replacement material in reconstructive procedures. The effectiveness of decellularization and the alterations to the ECM vary, depending on several factors, including the tissue source, composition and density. With an optimized decellularization process, decellularized scaffolds can preserve the spatial and temporal ECM microenvironment, which play an integral role in modulating cell migration, proliferation and differentiation. The exploration of a variety of decellularization protocols has led to mixed outcomes and comparisons between decellularization protocols could not attribute these differences to any single step in a multiple-step process. This study aimed to characterize the effects of each step of a multifactorial decellularization method on the scaffold structure and mechanical integrity of bovine pericardium. Each step of the decellularization process and the eff...

中文翻译：

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基于多因素洗涤剂的脱细胞过程对牛心包的顺序影响

去细胞化是获得细胞外基质支架 (ECM) 以用作重建程序中的替代材料的有前途的方法。去细胞化的有效性和对 ECM 的改变取决于几个因素，包括组织来源、组成和密度。通过优化的脱细胞过程，脱细胞支架可以保留时空 ECM 微环境，这在调节细胞迁移、增殖和分化中起着不可或缺的作用。对各种去细胞化协议的探索导致了不同的结果，去细胞化协议之间的比较不能将这些差异归因于多步骤过程中的任何一个步骤。本研究旨在表征多因素脱细胞方法的每个步骤对牛心包支架结构和机械完整性的影响。脱细胞过程的每一步和效果...