Wound healing has long been recognised as a major clinical challenge for which stablishing more effective wound therapies is necessary. The generation of metallic nanocomposites using biological compounds is emerging as a new promising strategy for this purpose. In this study, four metallic nanoparticles (NPs) with propolis extract (Ext) and one without propolis including ZnO/Ext, ZnO/Ag/Ext, ZnO/CuO/Ext, ZnO/Ag/CuO/Ext and ZnO/W were prepared by microwave method and assessed for their wound healing activity on excision experimental model of wounds in rats. The developed nanocomposites have been characterised by physico-chemical methods such as X-ray diffraction, scanning electron microscopy, diffuse reflectance UV–vis spectroscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis and Brunauer–Emmett–Teller analyses. The wounded animals treated with the NPs/Ext in five groups for 18 days. Every 6 days, for measuring wound closure rate, three samples of each group were examined for histopathological analysis. The prepared tissue sections were investigated by haematoxylin and Eosin stainings for the formation of epidermis, dermis and muscular and Masson's trichrome staining for the formation of collagen fibres. These findings toughly support the probability of using this new ZnO/Ag/Ext materials dressing for a wound care performance with significant effect compared to other NPs.

中文翻译：

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使用蜂胶提取物生物合成的 ZnO/CuO/Ag 纳米复合材料并涂在纱布上，用于伤口愈合应用

长期以来，伤口愈合一直被认为是一项主要的临床挑战，因此需要建立更有效的伤口治疗方法。使用生物化合物生成金属纳米复合材料正在成为一种新的有前途的策略。在这项研究中，制备了四种含有蜂胶提取物 (Ext) 和一种不含蜂胶的金属纳米粒子 (NPs)，包括 ZnO/Ext、ZnO/Ag/Ext、ZnO/CuO/Ext、ZnO/Ag/CuO/Ext 和 ZnO/W采用微波法在大鼠创面切除实验模型上评价其创面愈合活性。开发的纳米复合材料已通过物理化学方法进行表征，例如 X 射线衍射、扫描电子显微镜、漫反射紫外-可见光谱、傅里叶变换红外光谱、热重分析和 Brunauer-Emmett-Teller 分析。用 NPs/Ext 分五组治疗受伤动物 18 天。每 6 天，为了测量伤口闭合率，检查每组的三个样本进行组织病理学分析。制备的组织切片通过苏木精和伊红染色研究表皮、真皮和肌肉的形成，以及马森三色染色研究胶原纤维的形成。这些发现有力地支持了使用这种新型 ZnO/Ag/Ext 材料敷料来实现伤口护理性能的可能性，与其他 NPs 相比具有显着效果。制备的组织切片通过苏木精和伊红染色研究表皮、真皮和肌肉的形成，以及马森三色染色研究胶原纤维的形成。这些发现有力地支持了使用这种新型 ZnO/Ag/Ext 材料敷料来实现伤口护理性能的可能性，与其他 NPs 相比具有显着效果。制备的组织切片通过苏木精和伊红染色研究表皮、真皮和肌肉的形成，以及马森三色染色研究胶原纤维的形成。这些发现有力地支持了使用这种新型 ZnO/Ag/Ext 材料敷料来实现伤口护理性能的可能性，与其他 NPs 相比具有显着效果。