Gold nanoparticles (AuNPs) are commonly used in biosensors of various kinds. However, its application to extract DNA from cancer tissues has not been extensively studied. The purification of DNA from cancer tissues is an important step in diagnostic and therapeutic development. Almost, all cervical cancer cases are associated with high-risk human papillomavirus (HR-HPV) infection. Accurate viral diagnosis has so far relied on the extraction of adequate amounts of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples. Till now, no specific and sensitive DNA purification method has been introduced for the extraction of HR-HPV from FFPE tissue. Since the commercially available purification kits are not sensitive and specific enough for HR-HPV DNA targets, in this study, a DNA purification method was designed based on AuNPs to purify sufficient amounts of HR-HPV DNA from cervical cancer tissue samples. AuNPs were coated with a series of oligonucleotide probes to hybridize to specific DNA sequences of HR-HPV genotypes. Results showed that 733 out of 800 copies of type-specific HPV DNA were recovered with complete specificity, compared to 36 copies with a standard commercial kit (Qiagen FFPE). The high yield of DNA (91.6%) is the main advantage of the AuNPs-probe purification method.

中文翻译：

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从宫颈癌组织样本中提取人乳头瘤病毒基因型的高产量金纳米颗粒 DNA 分离方法

金纳米粒子（AuNPs）通常用于各种生物传感器。然而，它在从癌组织中提取 DNA 的应用尚未得到广泛研究。从癌组织中纯化 DNA 是诊断和治疗发展的重要一步。几乎所有宫颈癌病例都与高危人乳头瘤病毒 (HR-HPV) 感染有关。迄今为止，准确的病毒诊断依赖于从福尔马林固定、石蜡包埋 (FFPE) 组织样本中提取足量 DNA。到目前为止，还没有针对从 FFPE 组织中提取 HR-HPV 的特异性和灵敏的 DNA 纯化方法。由于市售的纯化试剂盒对 HR-HPV DNA 靶标的敏感性和特异性不够，因此在本研究中，基于AuNPs设计了一种DNA纯化方法，从宫颈癌组织样本中纯化足量的HR-HPV DNA。AuNPs 涂有一系列寡核苷酸探针，以与 HR-HPV 基因型的特定 DNA 序列杂交。结果显示，在 800 个类型特异性 HPV DNA 拷贝中，有 733 个完全特异性地被回收，而标准商业试剂盒 (Qiagen FFPE) 则为 36 个拷贝。DNA 的高产率 (91.6%) 是 AuNPs 探针纯化方法的主要优势。与标准商业试剂盒 (Qiagen FFPE) 的 36 个副本相比。DNA 的高产率 (91.6%) 是 AuNPs 探针纯化方法的主要优势。与标准商业试剂盒 (Qiagen FFPE) 的 36 个副本相比。DNA 的高产率 (91.6%) 是 AuNPs 探针纯化方法的主要优势。