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Transcriptomic changes in green bean pods against grey mould and white rot diseases via field application of chemical elicitor nanoparticles
IET Nanobiotechnology ( IF 2.3 ) Pub Date : 2020-10-05 , DOI: 10.1049/iet-nbt.2020.0004
Hoda A S El-Garhy 1 , Ahmed A Elsisi 2 , Shereen A Mohamed 1 , Osama M Morsy 3 , Gamal Osman 4 , Fayz A Abdel-Rahman 5
Affiliation  

The authors tested the efficacy of two salt nanoparticles (NPs), namely, copper dioxide (CuO) and tri-calcium phosphate [Ca 3 (PO 4 ) 2 ] to induce resistance in green bean pods against grey mould and white rot diseases caused by Botrytis cinerea and Sclerotinia sclerotiorum , respectively. High amounts of phytoalexins, kievitone, coumestrol, phaseollidin, 6-ά-hydroxyphaseollin, and phaseollin, were detected in naturally infected and artificially inoculated green bean pods in response to the tested NPs. Green bean plants treated in the field with CuO and Ca 3 (PO 4 ) 2 NPs had the highest mRNA quantity of all the studied defence genes, receptor-like kinase ( PvRK20 ), pathogenesis-related protein ( PR1 ), 1,3-β-D-glucanase ( pvgluc ), polygalacturonase inhibitor protein ( PvGIP ), and alpha-dioxygenase ( a-DOX ) than that of the control group. CuO NPs followed by Ca 3 (PO 4 ) 2 NPs at 0.15 mg ml −1 were the most potent in increasing the transcriptomic levels of pk20, DOX, PR1, PvGIP, and pvgluc. Field applications of both chemical elicitor NPs exhibited a non-genotoxic effect on the Paulista green bean DNA using eight ISSR primers. The field application of the studied NPs could effectively extend the shelf life of green bean pods by up to 21 days at 7 ± 1°C during marketing and export due to its potent effect against grey mould and white rot diseases.

中文翻译:

通过现场应用化学诱导子纳米粒子对绿豆荚抗灰霉病和白腐病的转录组学变化

作者测试了两种盐纳米颗粒 (NPs) 的功效,即二氧化铜 (CuO) 和磷酸三钙 [Ca 3 (PO 4 ) 2 ] 以诱导绿豆荚对灰霉病和白腐病引起的抗性灰霉病菌核盘菌 , 分别。响应测试的 NP,在自然感染和人工接种的绿豆豆荚中检测到大量植物抗毒素、kievitone、香豆雌酚、菜豆素、6-α-羟基菜豆素和菜豆素。在田间用 CuO 和 Ca 3 (PO 4 ) 2 NPs处理的绿豆植物在 所有研究的防御基因中具有最高的 mRNA 量,受体样激酶 ( PvRK20 ), 发病相关蛋白 ( 公关1 ), 1,3-β-D-葡聚糖酶 ( 聚葡萄糖 ), 多聚半乳糖醛酸酶抑制剂蛋白 ( PvGIP ) 和 α-双加氧酶 ( a-DOX ) 高于对照组。CuO NPs,其次是 Ca 3 (PO 4 ) 2 NPs,0.15 mg ml -1在增加 pk20、DOX、PR1、PvGIP 和 pvgluc 的转录组水平方面最有效。使用 8 种 ISSR 引物,两种化学诱导剂 NP 的现场应用对保利斯塔绿豆 DNA 表现出非遗传毒性作用。由于其对灰霉病和白腐病的有效作用,所研究的纳米颗粒的现场应用可以有效地将绿豆荚在销售和出口期间在 7 ± 1°C 下的保质期延长 21 天。
更新日期:2020-10-06
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