The objective of the current in-vitro study was to evaluate the biocompatibility of a new type of CAD/CAM scaffold for bone tissue engineering by using human cells. Porous lightweight titanium scaffolds and Bio-Oss® scaffolds as well as their eluates were used for incubation with human osteoblasts, fibroblasts and osteosarcoma cells. The cell viability was assessed by using fluorescein diazo-acetate propidium iodide staining. Cell proliferation and metabolism was examined by using MTT-, WST-Test and BrdU-ELISA tests. Scanning electron microscope was used for investigation of the cell adhesion behaviour. The number of devitalised cells in all treatment groups did not significantly deviate from the control group. According to MTT and WST results, the number of metabolically active cells was decreased by the eluates of both test groups with a more pronounced impact of the eluate from Bio-Oss®. The proliferation of the cells was inhibited by the addition of the eluates. Both scaffolds showed a partial surface coverage after 1 week and an extensive to complete coverage after 3 weeks. The CAD/CAM titanium scaffolds showed favourable biocompatibility compared to Bio-Oss® scaffolds in vitro. The opportunity of a defect-specific design and rapid prototyping by selective laser melting are relevant advantages in the field of bone tissue engineering and regenerative medicine.

中文翻译：

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用于骨组织工程的 CAD/CAM 支架：选择性激光熔化轻质钛的生物相容性研究

目前体外研究的目的是利用人体细胞评估用于骨组织工程的新型 CAD/CAM 支架的生物相容性。多孔轻质钛支架和 Bio-Oss® 支架及其洗脱液用于与人成骨细胞、成纤维细胞和骨肉瘤细胞一起孵育。通过使用荧光素重氮乙酸碘化丙啶染色评估细胞活力。通过使用 MTT-、WST-Test 和 BrdU-ELISA 测试检查细胞增殖和代谢。扫描电子显微镜用于研究细胞粘附行为。所有治疗组中失活细胞的数量与对照组没有显着差异。根据 MTT 和 WST 结果，两个测试组的洗脱液都减少了代谢活跃细胞的数量，Bio-Oss® 的洗脱液的影响更为显着。加入洗脱液可抑制细胞增殖。两种支架在 1 周后显示部分表面覆盖，在 3 周后显示广泛至完全覆盖。与 Bio-Oss® 支架相比，CAD/CAM 钛支架在体外显示出良好的生物相容性。通过选择性激光熔化进行缺陷特异性设计和快速原型制作的机会是骨组织工程和再生医学领域的相关优势。两种支架在 1 周后显示部分表面覆盖，在 3 周后显示广泛至完全覆盖。与 Bio-Oss® 支架相比，CAD/CAM 钛支架在体外显示出良好的生物相容性。通过选择性激光熔化进行缺陷特异性设计和快速原型制作的机会是骨组织工程和再生医学领域的相关优势。两种支架在 1 周后显示部分表面覆盖，在 3 周后显示广泛至完全覆盖。与 Bio-Oss® 支架相比，CAD/CAM 钛支架在体外显示出良好的生物相容性。通过选择性激光熔化进行缺陷特异性设计和快速原型制作的机会是骨组织工程和再生医学领域的相关优势。