This study aimed to develop sorafenib loaded magnetic microspheres for the treatment of hepatocellular carcinoma. To achieve this goal, superparamagnetic iron oxide nanoparticles (SPIONs) were synthesised and encapsulated in alginate microspheres together with an antineoplastic agent, sorafenib. In the study, firstly SPIONs were synthesised and characterised by dynamic light scattering, energy-dispersive X-ray spectroscopy, and scanning electron microscopy. Then, alginate-SPIONs microspheres were developed, and further characterised by electron spin resonance spectrometer and vibrating sample magnetometer. Besides the magnetic properties of SPIONs, alginate microspheres with SPIONs were also found to have magnetic properties. The potential use of microspheres in hyperthermia treatment was then investigated and an increase of about 4°C in the environment was found out. Drug release studies and cytotoxicity tests were performed after sorafenib was encapsulated into the magnetic microspheres. According to release studies, sorafenib has been released from microspheres for 8 h. Cytotoxicity tests showed that alginate-SPION-sorafenib microspheres were highly effective against cancerous cells and promising for cancer therapy.

中文翻译：

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用于治疗肝细胞癌的磁响应、负载索拉非尼的海藻酸盐微球

本研究旨在开发用于治疗肝细胞癌的载有索拉非尼的磁性微球。为了实现这一目标，我们合成了超顺磁性氧化铁纳米颗粒 (SPION)，并将其与抗肿瘤剂索拉非尼一起封装在海藻酸盐微球中。在这项研究中，首先合成了 SPIONs，并通过动态光散射、能量色散 X 射线光谱和扫描电子显微镜对其进行了表征。然后，开发了藻酸盐-SPIONs微球，并通过电子自旋共振光谱仪和振动样品磁强计进一步表征。除了 SPIONs 的磁性外，还发现含有 SPIONs 的海藻酸盐微球具有磁性。然后研究了微球在热疗治疗中的潜在用途，发现环境温度升高了约 4°C。在将索拉非尼封装到磁性微球中后进行药物释放研究和细胞毒性测试。根据释放研究，索拉非尼已从微球中释放 8 小时。细胞毒性试验表明，海藻酸盐-SPION-索拉非尼微球对癌细胞具有高效的抗癌作用，有望用于癌症治疗。