Electrochemical techniques such as impedance spectroscopy offer a non-invasive approach to monitor microorganisms in natural and engineered environments. Here, we present data on the use of microfabricated impedance spectroscopy sensors for the detection of microbes in icy environments. Under controlled laboratory settings, the effects of different cell concentrations of the Antarctic isolate Flavobacterium sp. ANT 11 on impedance spectroscopy was investigated at ambient temperature (22°C) and -10°C. Results show that varying cell concentrations of the bacterial isolate generate unique spectral responses that vary with temperature. Subsequent tests on the performance of microfabricated impedance sensors on natural microbial communities in icy environments in the Beartooth Mountains, WY, USA, confirmed laboratory findings. Different environmental samples at varying microbial concentrations in liquid, semi-frozen (slushy), or frozen states generated discrete impedance spectra. While caution is advised to generalize these results due to the potential contribution of unknown environmental variables, our data provide fertile territory for research in cryo-microbiology, as information on the applicability of impedance spectroscopy under frozen conditions is limited. [2020-0150]

中文翻译：

---

在冰冷环境中检测微生物活动的微传感器

阻抗谱等电化学技术提供了一种非侵入性方法来监测自然和工程环境中的微生物。在这里，我们提供了有关使用微制造阻抗光谱传感器检测冰冷环境中的微生物的数据。在受控实验室环境下，南极分离株黄杆菌属不同细胞浓度的影响。在环境温度 (22°C) 和 -10°C 下研究了阻抗谱上的 ANT 11。结果表明，细菌分离物的不同细胞浓度会产生随温度变化的独特光谱响应。随后对美国怀俄明州 Beartooth 山脉冰冷环境中天然微生物群落的微制造阻抗传感器的性能进行了测试，证实了实验室的发现。液体、半冷冻（泥泞）或冷冻状态中不同微生物浓度的不同环境样品产生离散阻抗谱。虽然由于未知环境变量的潜在贡献，建议谨慎概括这些结果，但我们的数据为低温微生物学研究提供了肥沃的领域，因为关于阻抗谱在冷冻条件下的适用性的信息有限。[2020-0150] 因为关于阻抗谱在冷冻条件下的适用性的信息是有限的。[2020-0150] 因为关于阻抗谱在冷冻条件下的适用性的信息是有限的。[2020-0150]