Somatic cell nuclear transfer (SCNT), which is an important procedure in cloning, has been conducted manually for decades. The operating efficiency drops sharply in batch SCNT because of the long-time observation under microscopy and the time-wasting traditional process. Though the operating time was reduced by robotic SCNT in previous studies, the traditional operating process was still used. In this article, we designed a new robotic batch SCNT process based on a microfluidic groove and two micropipettes in parallel. By using this new SCNT process, the operating area switching, objective lens conversing, and focusing on traditional SCNT process were eliminated, and oocyte localization was simplified, which saved much operating time. Experimental results showed that the new robotic batch process reduced about 50 s (41.7%) compared with the manual process (proposed 70 s versus manual 120 s). A success rate of 93.3% ( ${n} =30$ ) and a survival rate of 96.4% were achieved ( ${n}=28$ ), which were similar to manual process. The new robotic batch SCNT method demonstrated a high degree of efficiency and reproducibility. Note to Practitioners —This article presented a new robotic somatic cell nuclear transfer (SCNT) process. This new robotic SCNT process introduced a microfluidic groove for oocyte storage and two micropipettes for oocyte enucleation and oocyte injection, respectively. We save much operating time since the operating area switching, objective lens conversing, and focusing on traditional SCNT process were eliminated, and oocyte localization was simplified. Experimental results have demonstrated the efficiency and reproducibility of the new robotic SCNT process. This new robotic SCNT process has great potential for many other applications, e.g., ICSI, embryo microinjection, and cell biopsy. Commercialization of the proposed technology may lead to the improvement in SCNT industry. In current experiments, the somatic cells sometimes were injected at the same time, which led to the failure of the experiments. In the future, we will apply control algorithms to control the motion of multiple cells.

中文翻译：

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基于微流控槽的机器人批量体细胞核移植

体细胞核移植（SCNT）是克隆中的重要步骤，已经人工进行了数十年。由于在显微镜下的长时间观察和浪费时间的传统工艺，批次SCNT的操作效率急剧下降。尽管在以前的研究中机器人SCNT减少了操作时间，但仍使用传统的操作过程。在本文中，我们设计了一种新的机器人批处理SCNT工艺，该工艺基于微流控凹槽和并行的两个微量移液器。通过使用这种新的SCNT工艺，消除了手术区域切换，物镜转换和传统SCNT工艺的重点，简化了卵母细胞定位，从而节省了很多手术时间。实验结果表明，新的机器人批处理过程减少了约50 s（41。7％），与手动操作相比（建议的时间为70 s，而手动操作为120 s）。成功率为93.3％（ $ {n} = 30 $ ），生存率达到96.4％（ $ {n} = 28 $ ），类似于手动处理。新的自动批处理SCNT方法显示出高度的效率和可重复性。执业者注意 —本文介绍了一种新的机器人体细胞核移植（SCNT）工艺。这种新的机器人SCNT工艺引入了用于卵母细胞存储的微流控槽和分别用于卵母细胞去核和卵母细胞注射的两个微量移液器。由于省去了操作区域切换，物镜转换和传统SCNT处理的重点，简化了卵母细胞定位，因此节省了很多操作时间。实验结果证明了新型机器人SCNT工艺的效率和可重复性。这种新的自动SCNT工艺具有许多其他应用的巨大潜力，例如ICSI，胚胎显微注射和细胞活检。所提议技术的商业化可能导致SCNT行业的进步。在目前的实验中，有时会同时注射体细胞，导致实验失败。将来，我们将应用控制算法来控制多个单元的运动。