Red cabbage is a native vegetable of the Mediterranean region that represents one of the major sources of anthocyanins. The aim of this research is to evaluate the antioxidant capability and total polyphenol content (TPC) of a red cabbage extract and to compare acquired data with those from the same extract encapsulated in an acid-resistant capsule. The extract, which was qualitatively and quantitatively profiled by UHPLC-Q-Orbitrap HRMS analysis, contained a high content of anthocyanins and phenolic acids, whereas non-anthocyanin flavonoids were the less abundant compounds. An in vitro gastrointestinal digestion system was utilized to follow the extract’s metabolism in humans and to evaluate its colon bioaccessibility. Data obtained showed that during gastrointestinal digestion, the total polyphenol content of the extract digested in the acid-resistant capsule in the Pronase E stage resulted in a higher concentration value compared to the extract digested without the capsule. Reasonably, these results could be attributed to the metabolization process by human colonic microflora and to the genesis of metabolites with greater bioactivity and more beneficial effects. The use of red cabbage extract encapsulated in an acid-resistant capsule could improve the polyphenols’ bioaccessibility and be proposed as a red cabbage-based nutraceutical formulation for counteracting stress oxidative diseases.

中文翻译：

---

通过UHPLC-Q-Orbitrap HRMS化学分析红甘蓝提取物在体外胃肠道消化下的结肠生物可及性

红甘蓝是地中海地区的一种天然蔬菜，代表花青素的主要来源之一。这项研究的目的是评估红甘蓝提取物的抗氧化能力和总多酚含量（TPC），并将采集的数据与封装在耐酸胶囊中的相同提取物的数据进行比较。通过UHPLC-Q-Orbitrap HRMS分析定性和定量分析的提取物含有高含量的花青素和酚酸，而非花青素类黄酮含量较低。利用体外胃肠消化系统来追踪提取物在人体中的代谢，并评估其结肠的生物可及性。获得的数据表明，在消化过程中，与不使用胶囊消化的提取物相比，在Pronase E阶段在耐酸胶囊中消化的提取物的总多酚含量导致更高的浓度值。这些结果可以合理地归因于人结肠菌群的代谢过程以及具有更高生物活性和更有益作用的代谢产物的产生。封装在耐酸胶囊中的红甘蓝提取物的使用可以改善多酚的生物利用度，并被提议作为基于红甘蓝的保健品配方来应对应激性氧化疾病。这些结果可以归因于人结肠菌群的代谢过程以及具有更高生物活性和更有益作用的代谢产物的产生。封装在耐酸胶囊中的红甘蓝提取物的使用可以改善多酚的生物利用度，并被提议作为基于红甘蓝的保健品配方来应对应激性氧化疾病。这些结果可以归因于人结肠菌群的代谢过程以及具有更高生物活性和更有益作用的代谢产物的产生。封装在耐酸胶囊中的红甘蓝提取物的使用可以改善多酚的生物利用度，并被提议作为基于红甘蓝的保健品配方来应对应激性氧化疾病。