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Static Growth Promotes PrrF and 2-Alkyl-4(1H)-Quinolone Regulation of Type VI Secretion Protein Expression in Pseudomonas aeruginosa
Journal of Bacteriology ( IF 3.2 ) Pub Date : 2020-11-19 , DOI: 10.1128/jb.00416-20
Luke K. Brewer 1 , Weiliang Huang 1 , Brandy J. Hackert 1 , Maureen A. Kane 1 , Amanda G. Oglesby 1, 2
Affiliation  

Pseudomonas aeruginosa is an opportunistic pathogen that is frequently associated with both acute and chronic infections. P. aeruginosa possesses a complex regulatory network that modulates nutrient acquisition and virulence, but our knowledge of these networks is largely based on studies with shaking cultures, which are not likely representative of conditions during infection. Here, we provide proteomic, metabolic, and genetic evidence that regulation by iron, a critical metallonutrient, is altered in static P. aeruginosa cultures. Specifically, we observed a loss of iron-induced expression of proteins for oxidative phosphorylation, tricarboxylic acid (TCA) cycle metabolism under static conditions. Moreover, we identified type VI secretion as a target of iron regulation in P. aeruginosa cells under static but not shaking conditions, and we present evidence that this regulation occurs via PrrF small regulatory RNA (sRNA)-dependent production of 2-alkyl-4(1H)-quinolone metabolites. These results yield new iron regulation paradigms in an important opportunistic pathogen and highlight the need to redefine iron homeostasis in static microbial communities.

中文翻译:

静态增长促进铜绿假单胞菌VI型分泌蛋白表达的PrrF和2-烷基-4(1H)-喹诺酮调节。

铜绿假单胞菌是机会性病原体,经常与急性和慢性感染有关。铜绿假单胞菌拥有一个复杂的调节网络,可以调节营养物的获取和毒力,但是我们对这些网络的了解主要基于摇动培养的研究,这些研究不可能代表感染期间的状况。在这里,我们提供蛋白质组学,代谢和遗传学证据,证明静态铜绿假单胞菌中铁(一种重要的金属营养素)的调节作用发生了变化文化。具体来说,我们观察到铁在静态条件下氧化磷酸化,三羧酸(TCA)循环代谢中铁诱导的蛋白质表达的损失。此外,我们确定VI型分泌物是铜绿假单胞菌细胞在静态而不是摇动条件下铁调节的靶标,并且我们提供证据表明这种调节是通过依赖PrrF小调节RNA(sRNA)的2-烷基-4产生的(1 H)-喹诺酮代谢产物。这些结果在重要的机会病原体中产生了新的铁调节范例,并强调了在静态微生物群落中重新定义铁稳态的需要。
更新日期:2020-11-19
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