Objective: To explore the characteristics of oral and intestinal microbiota of pregnant women with hypothyroidism during pregnancy, and to find the correlations between the changes of flora and pregnancy outcome of pregnant women with hypothyroidism during pregnancy.Methods: In this study, oral and intestinal microbial composition was surveyed using 16S rRNA sequencing approach in 61 pregnant women (30 with hypothyroidism and 31 normal controls). Sequentially, we validated the differential microbial features using Quantitative Real-Time PCR (QPCR) approach of 10 randomly selected pregnant women (5 with hypothyroidism and 5 normal controls). Furthermore, general clinical data and serological indices were added to the analysis to examine the links between oral and intestinal microbiota and pregnancy outcomes.Results: The 16S rRNA results showed that the relative abundances of Gammaproteobacteria of pregnant women in the hypothyroidism group were higher than those in the control group, while the levels of Firmicutes in the control group were higher than those in the hypothyroidism group. The serum CRP level, the weight gain during pregnancy and the incidence of fetal distress in the hypothyroidism group were higher than control group. The QPCR results also showed the same changes of the intestinal in the two groups.Conclusion: There were significant differences in the oral and intestinal microbiota between pregnant women with hypothyroidism and normal pregnant women. The changes of microbiota is one of the factors influencing the occurrence and development of hypothyroidism during pregnancy.

中文翻译：

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甲状腺功能低下孕妇的口腔和肠道微生物特征及其与妊娠结局的关系

目的：探讨甲状腺功能减退症孕妇妊娠期口腔和肠道菌群的特征，探讨菌群变化与妊娠期甲状腺功能减退孕妇妊娠结局之间的相关性。使用16S rRNA测序方法对61名孕妇（30名甲状腺功能减退症和31名正常对照）进行了调查。随后，我们使用定量实时PCR（QPCR）方法对10名随机选择的孕妇（5名甲状腺功能减退症和5名正常对照）进行了验证，以区别微生物特征。此外，分析中还添加了一般临床数据和血清学指标，以检查口腔和肠道菌群与妊娠结局之间的联系。16S rRNA结果表明，甲状腺功能减退组孕妇γ丙酸杆菌的相对丰度高于对照组，而对照组的硬毛病水平高于甲状腺功能减退组。甲状腺功能减退组的血清CRP水平，妊娠期体重增加和胎儿窘迫发生率均高于对照组。QPCR结果也显示两组的肠道变化相同。结论：甲状腺功能减退症孕妇与正常孕妇的口腔和肠道菌群有显着差异。微生物群的变化是影响妊娠期甲状腺功能减退症发生和发展的因素之一。