This study presents a new type of biocompatible nanofiber based on poly(vinyl alcohol) (PVA) and carboxymethyl‐kappa‐carrageenan (CMKC) blends, produced with no generation of hazardous waste. The nanofibers are produced by electrospinning using PVA:CMKC blends with ratios of 1:0, 1:0.25, 1:0.4, 1:0.5, and 1:0.75 (w/w PVA:CMKC) in aqueous solution, followed by thermal crosslinking. The diameter of the fibers is in the nanometer scale and below 300 nm. Fourier transform infrared spectroscopy shows the presence of the carboxyl and sulfate groups in all the fibers with CMKC. The nanofibers from water‐soluble polymers are stabilized by thermal crosslinking. The incorporation of CMKC improves cytocompatibility, biodegradability, cell growth, and cell adhesion, compared to PVA nanofibers. Furthermore, the incorporation of CMKC modulates phenotype of human adipose‐derived stem cells (ADSCs). PVA/CMKC nanofibers enhance ADSC response to osteogenic differentiation signals and are therefore good candidates for application in tissue engineering to support stem cells.

中文翻译：

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由绿色工艺生产的聚（乙烯醇）/羧甲基-κ-角叉菜胶的生物相容性交联纳米纤维

本研究提出了一种基于聚乙烯醇 (PVA) 和羧甲基-κ-角叉菜胶 (CMKC) 混合物的新型生物相容性纳米纤维，生产时不产生危险废物。纳米纤维是通过使用 PVA:CMKC 共混物以 1:0、1:0.25、1:0.4、1:0.5 和 1:0.75 (w/w PVA:CMKC) 的比例在水溶液中静电纺丝，然后进行热交联而生产的. 纤维的直径为纳米级且低于300 nm。傅里叶变换红外光谱显示所有具有 CMKC 的纤维中都存在羧基和硫酸根。来自水溶性聚合物的纳米纤维通过热交联稳定。与 PVA 纳米纤维相比，CMKC 的掺入提高了细胞相容性、生物降解性、细胞生长和细胞粘附。此外，CMKC 的掺入调节人脂肪干细胞 (ADSC) 的表型。PVA/CMKC 纳米纤维增强了 ADSC 对成骨分化信号的反应，因此是组织工程中应用以支持干细胞的良好候选者。