As mixtures, deep eutectic solvent (DES) is designability. By adjusting the long alkyl chain hydrogen bond acceptors (HBAs) or hydrogen bond donors (HBDs), the DES displays surfactant characteristics and can form micelles. Hence, a novel, simple, facile and green natural organic acids capped copper nanoclusters (Aci-CuNCs) was synthesized and the spectrum behavior of Aci-CuNCs in DES micelles was researched. It was found that the surfactant-like DES can form micellar co-aggregation with Aci-CuNCs, resulting in the fluorescence (FL) intensive of Aci-CuNCs increase. Corresponding performance of spectral properties of Aci-CuNCs in DES medium were systematically studied by fourier transform infrared spectrometer, 3D FL spectroscopy, FL emission/excitation spectra, ultraviolet absorption spectroscopy. In the mechanism exploration part, on the one hand, the existence of micellar co-aggregation was confirmed by the conductivity, the mass effect of DES, dynamic light scattering and transmission electron microscopy. On the other hand, the influence of different kinds of DESs (types of HBAs/HBDs, molar ratio) and some possible factors (ionic strength and temperature) were discussed in detail to investigate the main driving forces for the formation of micellar co-aggregates. The results of mechanism exploration prove that the long alkyl chain of DES is amphiphilic which can form micellar co-aggregation with Aci-CuNCs through hydrogen bonding. The DES micelle provides Aci-CuNCs with a relatively stable and closed micro-environment which can effectively prevent collisions with water molecules and weakening of fluorescence intensity. On the basic of the above research, a “turn-off” fluorimetric method based on Aci-CuNCs in DES medium was applied for the determination of Fe³⁺. Under the optimum conditions, the assay worked in the Fe³⁺ concentration ranges from 1 to -20 μM and had a detection limit of 0.0374 μM. Method validation study illustrates the proposed system can provide a good accuracy, repeatability and stability conditions. Furthermore, the real sample analysis result demonstrates that no obvious matrix effect is found. As a consequence, the FL assays (Aci-CuNCs-based DES) composed of natural organic acid capped CuNCs and green solvent DES provides a simple, gentle and environmentally friendly method for the detection of iron ions.

作为混合物，深共熔溶剂（DES）具有可设计性。通过调节长烷基链氢键受体（HBA）或氢键供体（HBDs），DES可以显示表面活性剂特性并形成胶束。因此，合成了新颖，简单，易用，绿色的天然有机酸包覆的铜纳米团簇（Aci-CuNCs），研究了Aci-CuNCs在DES胶束中的光谱行为。发现表面活性剂样DES可以与Aci-CuNCs形成胶束共聚集，从而导致Aci-CuNCs的荧光（FL）强度增加。通过傅里叶变换红外光谱仪，3D FL光谱，FL发射/激发光谱，紫外吸收光谱等系统研究了Aci-CuNCs在DES介质中的光谱性能。一方面在机制探索部分，通过电导率，DES的质量效应，动态光散射和透射电子显微镜证实了胶束共聚集的存在。另一方面，详细讨论了不同种类的DES（HBA / HBD的类型，摩尔比）的影响以及一些可能的因素（离子强度和温度），以研究形成胶束共聚集体的主要驱动力。 。机理研究的结果证明，DES的长烷基链是两亲性的，可以通过氢键与Aci-CuNCs形成胶束共聚集。DES胶束为Aci-CuNC提供了相对稳定和封闭的微环境，可以有效防止与水分子发生碰撞并减弱荧光强度。在以上研究的基础上，³⁺。在最佳条件下，测定的Fe ³⁺浓度范围为1至-20μM，检出限为0.0374μM。方法验证研究表明，所提出的系统可以提供良好的准确性，可重复性和稳定性条件。此外，实际样品分析结果表明未发现明显的基质效应。因此，由天然有机酸封端的CuNC和绿色溶剂DES组成的FL分析（基于Aci-CuNCs的DES）为检测铁离子提供了一种简单，温和且环保的方法。