Osteosarcoma is the most common primary bone neoplasm in dogs and often involves the appendicular skeleton. We report the clinicopathological and prognostic features of appendicular osteosarcomas diagnosed in 153 dogs from 2008 to 2018. The survival data for 22 dogs that underwent surgery and chemotherapy were statistically compared with the clinical, histopathological and immunohistochemical data for correlation with prognosis. The affected dogs had a mean age of 9.1 years and a mean body weight of 33.4 kg. No sex predilection was detected, although the incidence was slightly higher in females (52%). Large mixed and purebred dogs were most commonly affected. Long bones were affected in 124 cases (92.5%; 124/134). The extremities affected within long bones were the metaphysis of the proximal humerus (29%), distal radius (17.7%), distal femur (15.3%) and proximal tibia (7.2%). Histologically, osteoblastic osteosarcoma was most commonly detected (56.9%), followed by chondroblastic (13.7%), telangiectatic (11.8%), fibroblastic (7.8%), giant cell-rich (5.2%) and poorly differentiated neoplasms (4.6%). Regional lymph nodes were available for analysis in 28 out of 70 excisional biopsies from amputated entire limbs. Metastases to regional lymph nodes were observed in 14.3% (4/28) of these cases. Distant metastases were found in 75% (39/52) of cases in which diagnostic imaging or necropsy was carried out. The lungs were the most common site of metastasis (87.2%; 34/39 cases). Weight, age, sex, affected limb or bone, histological classification, mitotic count or histological grade did not influence survival (P >0.05). Neoplasms located at proximal extremities (n = 7) of affected limbs had a tendency for a poorer prognosis than those at distal extremities (n = 15) (P = 0.06). For these cases, the 1-year survival rate was 14.3% compared with 40% for neoplasms at distal extremities. There was no significant difference in the survival rate when amputation (n = 16) or limb preservation (n = 6) techniques were employed with chemotherapy (P = 0.20). The survival period of dogs that had undergone limb amputation, or limb preservation surgery, and chemotherapy was 73–1,185 days, with a mean and median of 376 and 256 days, respectively. Mild to marked cytoplasmic immunolabelling of osteopontin was found in all cases but the intensity (P = 0.66) and percentage of neoplastic cells labelled (P = 0.49) did not influence survival.

骨肉瘤是犬最常见的原发性骨肿瘤，常累及四肢骨骼。我们报告了 2008 年至 2018 年在 153 只狗中诊断出的阑尾骨肉瘤的临床病理学和预后特征。 将接受手术和化疗的 22 只狗的生存数据与临床、组织病理学和免疫组织化学数据进行统计学比较，以确定与预后的相关性。受影响狗的平均年龄为 9.1 岁，平均体重为 33.4 公斤。没有检测到性别偏好，尽管女性的发病率略高（52%）。大型混合犬和纯种犬最常受到影响。124 例（92.5%；124/134）长骨受累。长骨内受影响的四肢是肱骨近端 (29%)、桡骨远端 (17.7%)、股骨远端 (15. 3%) 和胫骨近端 (7.2%)。在组织学上，最常检测到成骨细胞性骨肉瘤 (56.9%)，其次是成软骨细胞 (13.7%)、毛细血管扩张 (11.8%)、成纤维细胞 (7.8%)、富含巨细胞 (5.2%) 和分化差的肿瘤 (4.6%)。从截肢的整个肢体的 70 个切除活组织检查中，有 28 个区域淋巴结可用于分析。在这些病例中，14.3% (4/28) 观察到区域淋巴结转移。在进行诊断成像或尸检的病例中，75% (39/52) 发现远处转移。肺是最常见的转移部位（87.2%；34/39 例）。体重、年龄、性别、患肢或骨骼、组织学分类、有丝分裂计数或组织学分级不影响存活率（最常见的是成骨细胞骨肉瘤（56.9%），其次是成软骨细胞（13.7%）、毛细血管扩张（11.8%）、成纤维细胞（7.8%）、富含巨细胞（5.2%）和低分化肿瘤（4.6%）。从截肢的整个肢体的 70 个切除活组织检查中，有 28 个区域淋巴结可用于分析。在这些病例中，14.3% (4/28) 观察到区域淋巴结转移。在进行诊断成像或尸检的病例中，75% (39/52) 发现远处转移。肺是最常见的转移部位（87.2%；34/39 例）。体重、年龄、性别、患肢或骨骼、组织学分类、有丝分裂计数或组织学分级不影响存活率（最常见的是成骨细胞骨肉瘤（56.9%），其次是成软骨细胞（13.7%）、毛细血管扩张（11.8%）、成纤维细胞（7.8%）、富含巨细胞（5.2%）和低分化肿瘤（4.6%）。从截肢的整个肢体的 70 个切除活组织检查中，有 28 个区域淋巴结可用于分析。在这些病例中，14.3% (4/28) 观察到区域淋巴结转移。在进行诊断成像或尸检的病例中，75% (39/52) 发现远处转移。肺是最常见的转移部位（87.2%；34/39 例）。体重、年龄、性别、患肢或骨骼、组织学分类、有丝分裂计数或组织学分级不影响存活率（2%) 和低分化肿瘤 (4.6%)。从截肢的整个肢体的 70 个切除活组织检查中，有 28 个区域淋巴结可用于分析。在这些病例中，14.3% (4/28) 观察到区域淋巴结转移。在进行诊断成像或尸检的病例中，75% (39/52) 发现远处转移。肺是最常见的转移部位（87.2%；34/39 例）。体重、年龄、性别、患肢或骨骼、组织学分类、有丝分裂计数或组织学分级不影响存活率（2%) 和低分化肿瘤 (4.6%)。从截肢的整个肢体的 70 个切除活组织检查中，有 28 个区域淋巴结可用于分析。在这些病例中，14.3% (4/28) 观察到区域淋巴结转移。在进行诊断成像或尸检的病例中，75% (39/52) 发现远处转移。肺是最常见的转移部位（87.2%；34/39 例）。体重、年龄、性别、患肢或骨骼、组织学分类、有丝分裂计数或组织学分级不影响存活率（在进行诊断成像或尸检的病例中，75% (39/52) 发现远处转移。肺是最常见的转移部位（87.2%；34/39 例）。体重、年龄、性别、患肢或骨骼、组织学分类、有丝分裂计数或组织学分级不影响存活率（在进行诊断成像或尸检的病例中，75% (39/52) 发现远处转移。肺是最常见的转移部位（87.2%；34/39 例）。体重、年龄、性别、患肢或骨骼、组织学分类、有丝分裂计数或组织学分级不影响存活率（P  >0.05）。位于患肢近端（n = 7）的肿瘤比远端（n = 15）的肿瘤预后更差（P  = 0.06）。对于这些病例，1 年生存率为 14.3%，而四肢远端肿瘤的 1 年生存率为 40%。当截肢（n = 16）或肢体保留（n = 6）技术与化疗一起使用时，存活率没有显着差异（P  = 0.20）。接受截肢或保肢手术和化疗的狗的生存期为 73-1,185 天，平均和中位数分别为 376 天和 256 天。在所有病例中都发现了轻度至显着的骨桥蛋白细胞质免疫标记，但强度（P = 0.66）和标记的肿瘤细胞百分比（P  = 0.49）不影响存活率。