Fungal Genetics and Biology ( IF 3 ) Pub Date : 2020-10-06 , DOI: 10.1016/j.fgb.2020.103477 Lindsay K Caesar 1 , Neil L Kelleher 2 , Nancy P Keller 3
In 1990 the first fungal secondary metabolite biosynthetic gene was cloned in Aspergillus nidulans. Thirty years later, >30 biosynthetic gene clusters (BGCs) have been linked to specific natural products in this one fungal species. While impressive, over half of the BGCs in A. nidulans remain uncharacterized and their compounds structurally and functionally unknown. Here, we provide a comprehensive review of past advances that have enabled A. nidulans to rise to its current status as a natural product powerhouse focusing on the discovery and annotation of secondary metabolite clusters. From genome sequencing, heterologous expression, and metabolomics to CRISPR and epigenetic manipulations, we present a guided tour through the evolution of technologies developed and utilized in the last 30 years. These insights provide perspective to future efforts to fully unlock the biosynthetic potential of A. nidulans and, by extension, the potential of other filamentous fungi.
中文翻译:
在它发生的真菌中:历史和未来推动构巢曲霉作为天然产物研究的原型
1990 年第一个真菌次生代谢物生物合成基因被克隆到构巢曲霉中。三十年后,超过 30 个生物合成基因簇 (BGC) 与这一真菌物种中的特定天然产物有关。虽然令人印象深刻,但构巢曲霉中超过一半的 BGC仍未得到表征,其化合物的结构和功能未知。在这里,我们全面回顾了过去使A. nidulans上升到目前作为天然产物强国的地位,专注于次级代谢物簇的发现和注释。从基因组测序、异源表达和代谢组学到 CRISPR 和表观遗传操作,我们提供了过去 30 年来开发和使用的技术演变的导览。这些见解为未来充分释放构巢曲霉的生物合成潜力以及其他丝状真菌的潜力提供了前景。