SummaryRivulidae comprises a family of fish largely distributed in Brazil that includes 201 species, of which 125 are considered endangered. This fact emphasizes the need for development of conservation strategies including studies on genetics and reproduction. In this paper, we describe aspects of biology and reproduction of the rivuliid species Hypsolebias sertanejo. We outline the reproductive behaviour of this species under laboratory conditions, analyze ploidy status by flow cytometry, describe reproductive behaviour and performance and test dry and wet incubation of eggs. Although H. sertanejo showed well known patterns of reproductive behaviour, we verified many peculiarities inherent to its reproductive biology. As expected, most individuals were diploid (87.71%), however 14.29% were considered mosaics. Although no sterility was observed within mosaics, infertility of these fish was not fully evaluated. Hatching rate of the eggs collected was very low following both dry and wet incubation (5.04 and 3.79%, respectively). These results provide interesting information regarding the reproductive success of this species, and suggest that chromosomal abnormalities described may reduce the survival of H. sertanejo under natural conditions, limiting the perpetuation of this species, and emphasizing the need for more preservation efforts, including artificial propagation and gene banking.

中文翻译：

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生殖策略和染色体畸变影响 Rivuliid 鱼 Hypsolebias sertanejo 的生存

总结Rivulidae 包括一个主要分布在巴西的鱼类家族，其中包括 201 种，其中 125 种被认为是濒临灭绝的。这一事实强调了制定保护战略的必要性，包括遗传学和繁殖研究。在本文中，我们描述了 rivuliid 物种的生物学和繁殖方面荸荠. 我们概述了该物种在实验室条件下的繁殖行为，通过流式细胞术分析倍性状态，描述繁殖行为和性能，并测试鸡蛋的干孵化和湿孵化。虽然H.塞塔尼霍展示了众所周知的生殖行为模式，我们验证了其生殖生物学固有的许多特性。正如预期的那样，大多数个体是二倍体（87.71%），但是 14.29% 被认为是马赛克。虽然在马赛克中没有观察到不育，但这些鱼的不育没有得到充分评估。在干孵化和湿孵化之后，收集到的卵的孵化率非常低（分别为 5.04% 和 3.79%）。这些结果提供了有关该物种繁殖成功的有趣信息，并表明所描述的染色体异常可能会降低该物种的存活率。H.塞塔尼霍在自然条件下，限制该物种的延续，并强调需要更多的保护工作，包括人工繁殖和基因库。