ABSTRACT

To elucidate the underlying mechanism of secretory leukocyte protease inhibitor (SLPI)-induced cell migration, we compared SLPI-deleted human gingival carcinoma Ca9-22 (ΔSLPI) cells and original (wild-type: wt) Ca9-22 cells using several microscopic imaging methods and gene expression analysis. Our results indicated reduced migration of ΔSLPI cells compared to wtCa9-22 cells. The lamellipodia/dorsal ruffles were smaller and moved slower in ΔSLPI cells compared to wtCa9-22 cells. Furthermore, well-developed intermediate filament bundles were observed at the desmosome junction of ΔSLPI cells. In addition, Galectin4 was strongly expressed in ΔSLPI cells, and its forced expression suppressed migration of wtCa9-22 cells. Taken together, SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role.

摘要

为了阐明分泌性白细胞蛋白酶抑制剂 (SLPI) 诱导细胞迁移的潜在机制，我们使用几种显微成像比较了 SLPI 缺失的人牙龈癌 Ca9-22 (ΔSLPI) 细胞和原始（野生型：wt）Ca9-22 细胞方法和基因表达分析。我们的结果表明，与 wtCa9-22 细胞相比，ΔSLPI 细胞的迁移减少。与 wtCa9-22 细胞相比，ΔSLPI 细胞中的片状伪足/背褶皱更小且移动更慢。此外，在 ΔSLPI 细胞的桥粒连接处观察到发育良好的中间丝束。此外，半乳糖凝集素4在 ΔSLPI 细胞中强烈表达，其强制表达抑制了 wtCa9-22 细胞的迁移。总之，SLPI 通过调节片状伪足/褶皱和桥粒促进细胞迁移，其中 Galectin4 发挥重要作用。