ABSTRACT Dehydrins (DHNs) belong to group Ⅱ late embryogenesis abundant (LEA) proteins which perform multiple functions in plants during stress conditions. Both K- and S-segments are conserved domains in the dehydrin protein family; however, there are only a few in vivo functional studies for these two conserved segments. In this study, the DHN gene wzy1-2 was isolated from Triticum aestivum and its K-/S-segment-truncated derivatives were generated. In order to explore the biological function of these two conserved fragments, subcellular localization and dimerization detection assays were performed for the K-/S-segment-truncated derivatives. Results of GFP fusion and bimolecular fluorescence complementation (BiFC) assays indicated that WZY1-2 localized to nucleus as a homologous dimer. The S-segment partially regulated the nuclear localization of WZY1-2 but did not affect its dimerization, while the K-segment influenced neither the dimer formation nor the subcellular localization.

中文翻译：

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K-/S-片段对小麦脱水蛋白WZY1-2亚细胞定位和二聚化的影响

摘要 脱水蛋白 (DHNs) 属于 Ⅱ 组晚期胚胎发生丰富 (LEA) 蛋白，在胁迫条件下在植物中发挥多种功能。K-段和S-段都是脱水蛋白家族中的保守结构域；然而，这两个保守片段的体内功能研究很少。在这项研究中，DHN 基因 wzy1-2 是从小麦中分离出来的，并产生了其 K-/S-段截断的衍生物。为了探索这两个保守片段的生物学功能，对 K-/S-段截断的衍生物进行了亚细胞定位和二聚化检测分析。GFP 融合和双分子荧光互补 (BiFC) 测定的结果表明 WZY1-2 作为同源二聚体定位于细胞核。