Abstract

Phytochemical investigation of Adiantum flabellulatum L. led to the isolation of four natural compounds, including a novel unsaturated fatty acid with a cyclopropane moiety, i.e. (S,E)-7-(2-octylcyclopropylidene)heptanoic acid (1), together with three known compounds, isoadiantol B (2), stigmast-4-en-6β-ol-3-one (3), β-sitosterol (4). Compound 3 was isolated from the A. flabellulatum L. for the first time. The structure of 1 was elucidated following a comprehensive analysis of spectroscopic analyses including MS, 1 D and 2 D NMR, and by a mass spectrometry experiment of the dimethyl disulfide (DMDS) adduct, while the known compounds were identified by comparisons with those reported in the literature. Enzyme evaluation of 1 indicated this compound possesses anti- protein tyrosine phosphatase (PTP1B) activity with an IC₅₀ value of 6.99 ± 0.41 μM in vitro.

摘要

Adiantum flabellulatum L.的植物化学研究导致分离出四种天然化合物，包括一种具有环丙烷部分的新型不饱和脂肪酸，即 ( S , E )-7-(2-辛基亚环丙基)庚酸 ( 1 )，以及三种已知化合物，isoadiantol B ( 2 ), stigmast-4-en-6 β -ol -3-one ( 3 ), β-谷甾醇( 4 )。化合物3为首次从A. flabellulatum L.中分离得到。1的结构在对包括 MS、1D 和 2D NMR 在内的光谱分析的综合分析以及二甲基二硫 (DMDS) 加合物的质谱实验进行了全面分析后阐明了这些化合物，而通过与文献中报道的化合物进行比较来鉴定已知化合物。1的酶评估表明该化合物具有抗蛋白酪氨酸磷酸酶 (PTP1B) 活性，体外IC ₅₀值为 6.99 ± 0.41 μM 。