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Determination of mold contamination using ergosterol imprinted particles
Biotechnology Progress ( IF 2.9 ) Pub Date : 2020-10-05 , DOI: 10.1002/btpr.3089
Hatice İmge Oktay Başeğmez 1 , Gözde Baydemir Peşint 1 , Mustafa Nergiz 1 , Okan Zenger 1
Affiliation  

Ergosterol is a key biochemical marker for fungal mycelial growth. In this study, molecularly ergosterol imprinted particles (Erg‐MIPs) were newly synthesized for the selective detection of ergosterol in mold samples. Erg‐MIPs were characterized via scanning electron microscopy, swelling studies, and surface area measurements. Maximum selective ergosterol adsorption achieved as 28.50 mg/g Erg‐MIP. Selectivity studies showed that Erg‐MIPs adsorbed Erg 2.01 and 3.27 times higher than that of cholesterol and stigmasterol, respectively. Erg adsorption from Aspergillus niger was found as 23.87 mg/g. Reusability of Erg‐MIPs was studied and decrease in Erg adsorption capacity of the particles was negligible (3%). Erg‐MIPs are good affinity materials for the selective Erg detection from food samples, prior to use in food industry.

中文翻译:

使用麦角甾醇印迹颗粒测定模具污染

麦角甾醇是真菌菌丝体生长的关键生化标志物。在这项研究中,新合成了分子麦角甾醇印迹颗粒 (Erg-MIPs),用于选择性检测霉菌样品中的麦角甾醇。Erg-MIP 通过扫描电子显微镜、溶胀研究和表面积测量进行了表征。最大选择性麦角甾醇吸附达到 28.50 mg/g Erg-MIP。选择性研究表明,Erg-MIP 对 Erg 的吸附量分别是胆固醇和豆甾醇的 2.01 倍和 3.27 倍。黑曲霉的 Erg 吸附发现为 23.87 毫克/克。研究了 Erg-MIP 的可重复使用性,并且颗粒对 Erg 吸附能力的降低可以忽略不计(3%)。在用于食品工业之前,Erg-MIP 是用于从食品样品中选择性检测 Erg 的良好亲和材料。
更新日期:2020-10-05
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