The luminescent bacteria inhibition test using Aliivibrio fischeri is a well‐established method to determine the aquatic toxicity of soluble chemicals. More precisely, the effective concentration (EC₅₀) after 15 and 30 min is determined in this test. The inhibition of natural bioluminescence of these bacteria gives a first idea of the toxicity of compounds towards some aquatic organisms. It is a cost and time efficient experimental method, which does not involve animals. In this contribution, the experimental set up, comparability with other measurements, and results of recently described compounds is presented. Different types of energetic materials such as coordination (e. g. [Cu(dtp)₃](ClO₄)₂ and [Fe(MTZ)₆](ClO₄)₂), neutral (e. g. azidoethanol, 1‐ and 2‐aminotetrazole) and ionic (e. g. polynitropyrazolates and PETNC salts) compounds were investigated and compared to commonly used materials, like RDX, ammonium perchlorate (AP) and azide salts. Furthermore, different substitution patterns and energetic functionalities such as azido‐, nitro‐ and nitramino‐groups were investigated.

中文翻译：

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使用发光细菌抑制试验评估含能材料的毒性

使用费氏Aliivibrio fischeri进行的发光细菌抑制测试是一种确定可溶性化学物质的水生毒性的公认方法。更准确地说，在此测试中确定15和30分钟后的有效浓度（EC ₅₀）。这些细菌天然生物发光的抑制给出了化合物对某些水生生物毒性的第一个想法。这是一种省时省钱的实验方法，不涉及动物。在这项贡献中，介绍了实验装置，与其他测量方法的可比性以及最近描述的化合物的结果。不同类型的高能材料，例如配位化合物（例如[Cu（dtp）₃ ]（ClO ₄）₂和[Fe（MTZ）₆ ]（ClO ₄）₂），中性（例如叠氮乙醇，1-和2-氨基四唑）和离子（例如多硝基吡唑酸盐和PETNC盐）化合物进行了研究，并与常用化合物进行了比较材料，例如RDX，高氯酸铵（AP）和叠氮化物盐。此外，还研究了不同的取代模式和高能功能，例如叠氮基，硝基和硝基氨基。