Colorectal cancer (CRC) is one of the most common malignant tumors in the world. In this study, the Caco‐2 in vitro cell model was used to study the effect and mechanism of Aronia melanocarpa (Michx.) Elliott anthocyanins (AMA) on colon cancer. The experimental results showed that the binding energy of anthocyanins on β‐catenin was in the range of −5.92 to 4.95 kcal/mol, with good low energy parameters and binding positions. AMA can inhibit cell proliferation and cause cell cycle arrest. RT‐PCR and Western blot results showed that AMA can reduce cytoplasmic β‐catenin and inhibit the expression of related proteins in Wnt/β‐catenin signaling pathway. This study revealed the AMA inhibitory effect and mechanism of malignant biological behavior of Caco‐2 cells, in order to provide theoretical basis for the prevention and treatment of colon cancer by Aronia melanocarpa (Michx.) Elliott.

中文翻译：

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黑果树花青素通过 Wnt/β-catenin 信号通路诱导 Caco-2 细胞凋亡

结直肠癌（CRC）是世界上最常见的恶性肿瘤之一。本研究采用 Caco-2 体外细胞模型研究黑果楝树 (Aronia melanocarpa (Michx.) Elliott 花青素 (AMA) 对结肠癌的作用及机制)。实验结果表明，花青素对β-catenin的结合能在-5.92～4.95 kcal/mol范围内，具有良好的低能参数和结合位置。AMA 可以抑制细胞增殖并导致细胞周期停滞。RT-PCR和Western blot结果表明AMA可以降低细胞质β-catenin并抑制Wnt/β-catenin信号通路相关蛋白的表达。本研究揭示了 Caco-2 细胞恶性生物学行为的 AMA 抑制作用及机制，