Accurate intraoperative tissue identification is critical to tumor surgery. However, conventional methods are labor‐ and time‐intensive, which greatly delay the intraoperative decision‐making. Herein, a matrix metalloproteinase (MMP)14‐activated NIR‐II nanoprobe (A&MMP@Ag₂S‐AF7P) is presented for rapid unperturbed‐tissue analysis for ex vivo and in vivo neuroblastoma diagnosis. A&MMP@Ag₂S‐AF7P displays negligible fluorescence in normal tissues but is activated quickly by inhibiting the fluorescence resonance energy transfer (FRET) between Ag₂S QDs and A1094 mediated by MMP14 overexpressed in neuroblastoma; meanwhile, the exposure of the membrane penetrating peptide R9 (TAT‐peptide) results in efficient internalization of nanoprobes in the cancer cells, providing superior tumor‐to‐normal (T/N) tissue ratio. Instant illumination of the lesion and well‐defined tumor margins make the nanoprobes a suitable rapid diagnostic reagent for cancer surgical or tissue biopsy procedures.

中文翻译：

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使用酶激活的NIR-II纳米探针对术中癌症进行快速无扰动组织分析

准确的术中组织识别对于肿瘤手术至关重要。但是，传统方法费时费力，大大延迟了术中决策的时间。本文介绍了一种基质金属蛋白酶（MMP）14活化的NIR-II纳米探针（A＆MMP @ Ag ₂ S-AF7P），用于快速无扰动的组织分析，用于离体和体内神经母细胞瘤的诊断。A＆MMP @ Ag ₂ S‐AF7P在正常组织中显示的荧光可忽略不计，但通过抑制Ag ₂之间的荧光共振能量转移（FRET）可以快速激活MMP14介导的S QD和A1094在神经母细胞瘤中过表达；同时，膜穿透肽R9（TAT肽）的暴露可导致癌细胞内纳米探针的有效内在化，从而提供优异的肿瘤与正常（T / N）组织比率。病变的即时照明和明确的肿瘤切缘使纳米探针成为用于癌症外科手术或组织活检程序的合适快速诊断试剂。