Research question

Are triglyceride fatty acids in the follicular fluid associated with either follicular fluid phospholipid fatty acids or IVF outcomes and, if so, how are they associated?

Design

In a prospective cross-sectional study, 70 women undergoing intracytoplasmic sperm injection were recruited. Follicular fluid phospholipids and triglycerides were separated by thin-layer chromatography. Fatty acids were measured using gas-liquid chromatography and flame ionization detection system.

Results

Significant differences in fatty acid composition were observed between follicular fluid phospholipid and triglyceride fractions. Phospholipid stearic acid and n-3 polyunsaturated fatty acids, particularly alpha-linolenic acid, were negatively associated with the number of mature oocytes and cleaved embryos, whereas arachidonic acid was in direct correlation with cleavage rate per IVF cycle (β = 0.325, P = 0.022). In the case of triglyceride fraction, total monounsaturated fatty acids, oleic acid in particular, displayed significantly positive associations with the number of oocytes (β = 0.261, P = 0.043) and embryos (β = 0.310, P = 0.018). Furthermore, cleavage rate correlated inversely with palmitic acid (β = –0.359, P = 0.007) and directly with pentadecanoic acid (β = 0.378, P = 0.005). Most of these associations, however, were not independent of predictive fatty acids belonging to phospholipid fraction, according to multivariate analysis.

Conclusions

Fatty acid compositions of phospholipid and triglyceride fractions from human follicular fluid differentially correlate with IVF cycle parameters.

中文翻译：

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卵泡液磷脂和甘油三酯的脂肪酸显示出与试管婴儿结果的明显关联

研究问题

卵泡液中的甘油三酯脂肪酸是否与卵泡液磷脂脂肪酸或 IVF 结果相关，如果是，它们是如何相关的？

设计

在一项前瞻性横断面研究中，招募了 70 名接受卵胞浆内单精子注射的女性。通过薄层色谱分离卵泡液磷脂和甘油三酯。使用气液色谱法和火焰离子化检测系统测量脂肪酸。

结果

在卵泡液磷脂和甘油三酯部分之间观察到脂肪酸组成的显着差异。磷脂硬脂酸和 n-3 多不饱和脂肪酸，尤其是 α-亚麻酸，与成熟卵母细胞和卵裂胚胎的数量呈负相关，而花生四烯酸与每个 IVF 周期的卵裂率直接相关（β = 0.325，P  = 0.022）。在甘油三酯部分的情况下，总单不饱和脂肪酸，特别是油酸，与卵母细胞 (β = 0.261, P  = 0.043) 和胚胎 (β = 0.310, P  = 0.018)的数量呈显着正相关。此外，裂解率与棕榈酸呈负相关（β = –0.359，P = 0.007) 和直接使用十五烷酸 (β = 0.378, P  = 0.005)。然而，根据多变量分析，这些关联中的大多数并不独立于属于磷脂部分的预测性脂肪酸。

结论

来自人类卵泡液的磷脂和甘油三酯组分的脂肪酸组成与 IVF 周期参数差异相关。