Peroxiredoxins are a group of thiol-specific antioxidant proteins that take six isoforms in vertebrates and allow the innate immune system to sense and detoxify reactive oxygen species. In this study, we identified and characterized the perxiredoxin-1 (SsPrdx1) cDNA sequence from the rockfish, Sebastes schlegelii. In silico analysis revealed that SsPrdx1 contained a 594 bp long open reading frame (ORF) encoding a protein of 198 amino acids, with a predicted molecular weight and theoretical isoelectric point of 21.97 kDa and 6.30, respectively. The SsPrdx1 gene comprised six exons linked by five introns, while peroxiredoxin signature motifs were found in the highly conserved third, fourth, and fifth exons. Phylogenetic analysis and sequence alignment suggested that SsPrdx1 is evolutionarily conserved and that its most closely related counterpart is Salarias fasciatus. Recombinant SsPrdx1 (rSsPrdx1) displayed supercoiled DNA protection and insulin disulfide reduction activities in a concentration-dependent manner, while cells transiently transfected with pcDNA3.1 (+)/SsPrdx1 exhibited significant cytoprotective effects under oxidative stress and wound healing activity. SsPrdx1 transcripts were constitutively expressed under normal physiological conditions, with the highest expression observed in the blood. Moreover, SsPrdx1 expression increased in the blood, spleen, and liver following immune provocation by LPS, poly I:C, and Streptococcus iniae injection. Thus, this study provides insights into the role of SsPrdx1 in rockfish immune protection.

Peroxiredoxins是一组硫醇特异性抗氧化剂蛋白，在脊椎动物中具有六种亚型，可让先天免疫系统感应和解毒活性氧。在这项研究中，我们从石斑鱼Sebastes schlegelii中鉴定和鉴定了perxiredoxin-1（SsPrdx1）cDNA序列。在计算机分析中发现，SsPrdx1含有594 bp长的开放阅读框（ORF），编码198个氨基酸的蛋白质，预测的分子量和理论等电点分别为21.97 kDa和6.30。该SsPrdx1该基因包含由五个内含子连接的六个外显子，而在高度保守的第三，第四和第五个外显子中发现了过氧化物酶原签名基序。系统发育分析和序列比对表明，SsPrdx1是进化保守的，其最密切相关的对应物是Salarias fasciatus。重组SsPrdx1（rSsPrdx1）以浓度依赖的方式显示超螺旋DNA保护和胰岛素二硫化物还原活性，而瞬时转染了pcDNA3.1（+）/ SsPrdx1的细胞在氧化应激和伤口愈合活性下表现出显着的细胞保护作用。SsPrdx1转录本在正常生理条件下组成性表达，在血液中观察到最高表达。此外，SsPrdx1表达在血液，脾增大，并按照LPS，聚I免疫挑衅肝：C，和海豚链球菌注射。因此，本研究提供了对SsPrdx1在石鱼免疫保护中的作用的见解。