Salt extraction and Ultrafiltration membrane (UFM) separation technology of bovine bone marrow protein (BBMP) were optimized, and the structures of proteins and peptides, as well as biological activities were evaluated. The maximum protein content and yield obtained were 57.40 mg/mL, 56.80% respectively by salt extraction under the following conditions: concentration 0.5 mol/L, extraction of 2 times per 3 h; temperature, 45 °C. Maximum membrane flux and protein content reached to 25.29 L/(m² h), 76.9 mg/mL using UFMs method optimized by the response surface methodology (RSM) at 10 kDa membrane, operation pressure 0.20 MPa; concentration rate 5, and temperature 40 °C. Among them, the 3 kDa permeate part has the highest radical scavenging capacity against DPPH and hydroxyl free radicals (41 ± 0.20%, 53 ± 0.35%, respectively). These isolated parts were characterized by Fourier transform infrared spectrometry (FT-IR), scanning electron microscopy (SEM), atomic force microscope (AFM), sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), and Liquid chromatography-Mass spectrometer-Mass spectrometer (LC–MS/MS). The key functional groups, molecular weight, and mass data accordance with the UFM separation results. Therefore, the separation of protein and peptides with different molecular weights can be achieved by UFM. This study can provide technical support for the full utilization and scientific application of BBM resources, as well as the technical and theoretical foundation for the development of peptides for potential health foods or drugs supplementary materials.

优化了牛骨髓蛋白（BBMP）的盐提取和超滤膜（UFM）分离技术，并评估了蛋白质和肽的结构以及生物学活性。在以下条件下通过盐提取获得的最大蛋白质含量和最大产量分别为57.40 mg / mL，56.80％：浓度0.5 mol / L，每3 h提取2次；温度为45°C。最大膜通量和蛋白质含量达到25.29 L /（m ²h），采用UFMs方法，通过响应面法（RSM）优化，在10 kDa膜上，操作压力为0.20 MPa，为76.9 mg / mL；浓缩率5，温度40°C。其中，3 kDa的渗透物对DPPH和羟基自由基的清除能力最高（分别为41±0.20％，53±0.35％）。这些分离的部分通过傅里叶变换红外光谱（FT-IR），扫描电子显微镜（SEM），原子力显微镜（AFM），十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和液相色谱-质谱仪进行表征。质谱仪（LC-MS / MS）。关键的官能团，分子量和质量数据符合UFM分离结果。因此，可以通过UFM分离具有不同分子量的蛋白质和肽。