The aim of this study was to investigate the effect of luteolin (Lu) on dextran sodium sulfate (DSS)–induced colitis in mice. Mice spleen was weighed. The length of colon was measured. H&E staining was used to observe the pathological changes of colon in mice. Superoxide dismutase (SOD) and malondialdehyde (MDA) in serum and intestine of mice were detected by commercial kits. Serum and intestinal cytokines were detected by ELISA kits. The expression of HMGB1 mRNA was detected by real-time PCR. The expression of HMGB1-TLR-NF-κB pathway was detected by Western blot. The level of HMGB1 was detected by immunohistochemistry. The results showed that Lu significantly increased the colon length/body weight ratio and significantly decreased the spleen weight/body weight ratio. Lu significantly increased serum and intestinal SOD levels and decreased MDA levels. Lu significantly increased serum and intestinal cytokine levels in mice. qPCR and immunohistochemistry results showed that Lu significantly reduced HMGB1 mRNA level and protein level. In addition, Lu significantly reduced the expression of HMGB1-TLR-NF-κB signaling pathway protein of intestine in mice. In conclusion, Lu significantly reduced and alleviated DSS-induced colitis in mice, and the mechanism was related to the regulation of intestinal HMGB1-TLR-NF-κB signaling pathway in mice.

本研究的目的是研究木犀草素 (Lu) 对葡聚糖硫酸钠 (DSS) 诱导的小鼠结肠炎的影响。称重小鼠脾脏。测量结肠的长度。H&E染色观察小鼠结肠病理变化。采用市售试剂盒检测小鼠血清和肠道中的超氧化物歧化酶（SOD）和丙二醛（MDA）。ELISA试剂盒检测血清和肠道细胞因子。实时荧光定量PCR检测HMGB1 mRNA的表达。Western blot检测HMGB1-TLR-NF-κB通路的表达。通过免疫组织化学检测HMGB1的水平。结果表明，Lu显着增加了结肠长度/体重比，并显着降低了脾重/体重比。Lu 显着增加血清和肠道 SOD 水平并降低 MDA 水平。Lu 显着增加了小鼠的血清和肠道细胞因子水平。qPCR和免疫组化结果表明，Lu显着降低了HMGB1 mRNA水平和蛋白质水平。此外，Lu显着降低小鼠肠道HMGB1-TLR-NF-κB信号通路蛋白的表达。综上所述，Lu可显着减轻和减轻DSS诱发的小鼠结肠炎，其机制与小鼠肠道HMGB1-TLR-NF-κB信号通路的调节有关。Lu显着降低小鼠肠道HMGB1-TLR-NF-κB信号通路蛋白的表达。综上所述，Lu可显着减轻和减轻DSS诱发的小鼠结肠炎，其机制与小鼠肠道HMGB1-TLR-NF-κB信号通路的调节有关。Lu显着降低小鼠肠道HMGB1-TLR-NF-κB信号通路蛋白的表达。综上所述，Lu可显着减轻和减轻DSS诱发的小鼠结肠炎，其机制与小鼠肠道HMGB1-TLR-NF-κB信号通路的调节有关。