An integrated process to increase the yield of incomplete degradation products of galactomannan (GalM) especially for galactomanno-oligosaccharides (GalMOS) was suggested. Trichoderma reesei employed Avicel or GalMOS as a carbon source to produce β-mannanase or α-galactosidase independently, with a result of 3.78 ± 0.12 U/mL of β-mannanase activity and 2.45 ± 0.06 U/mL of α-galactosidase activity which were obtained, respectively. GalM in Sesbania seed was hydrolyzed simultaneously by a mixture of crude enzyme with β-mannanase and α-galactosidase at a dosage of 20 U/g GalM and 15 U/g GalM, respectively; the yields of incomplete degradation products of GalM (IDP-GalM) and GalMOS were 78.84% ± 3.14% and 30.94% ± 0.38%, respectively, which was beneficial to improve the biological activity of the incomplete degradation products. The role of α-galactosidase addition in mixture enzymes is to remove the galactose substituents from mannan backbone of GalM and alleviate the steric hindrance of β-mannanase hydrolysis.

提出了增加半乳甘露聚糖（GalM）的不完全降解产物的收率的综合方法，特别是对于半乳甘露寡糖（GalMOS）。里氏木霉使用Avicel或GalMOS作为碳源独立生产β-甘露聚糖酶或α-半乳糖苷酶，结果获得了3.78±0.12 U / mL的β-甘露聚糖酶活性和2.45±0.06 U / mL的α-半乳糖苷酶活性，分别。用粗酶与β-甘露聚糖酶和α-半乳糖苷酶的混合物分别以20 U / g GalM和15 U / g GalM的混合物同时水解塞巴尼亚种子中的GalM；GalM（IDP-GalM）和GalMOS的不完全降解产物的产率分别为78.84％±3.14％和30.94％±0.38％，这有利于提高不完全降解产物的生物活性。在混合酶中添加α-半乳糖苷酶的作用是从GalM的甘露聚糖主链上去除半乳糖取代基并减轻β-甘露聚糖酶水解的空间位阻。