Pathogenic bacteria cause significant economic damages in agriculture. The detection of such bacteria is considered as a continual interest for plant pathologists to prevent disease dissemination. Pseudomonas syringae pv. syringae is one of the most important bacterial pathogens infecting yield and quality of stone fruits throughout the world. Biochemical assays such as a LOPAT and GATTa are common methods to detect this pathogen. Serological tests and culturing on King’s B selective medium also used to isolate this bacterium. Selective media is composed of specific and effective ingredients to inhibit the growth of certain species of microbes in a mixed culture while allowing others to grow. These are used for the growth of only selected microorganisms. King’s B medium can be used as a general medium for the non-selective isolation cultivation and pigment production of Pseudomonas species from foods, cosmetic samples, plants, etc. Nevertheless, the mentioned methods are not enough accurate to differentiate the strains. On the other hand, PCR-based techniques are sensitive and efficient in detecting plant diseases. However, these techniques are not practicable for those researchers who do not have access to a thermal cycler. We have used loop-mediated isothermal amplification to couple with a target. The amplification of syrD gene using loop and bumper primers can be used to prevent disease dissemination. The outcome of this investigation indicated more sensitivity of LAMP in comparison to PCR. The direct addition of SYBR Gold in microtube is more sensitive than gel in both LAMP and PCR byproducts so we can eliminate gel electrophoresis, while the LAMP showed high sensitivity and high specificity in comparison to results obtained by cultivation. The described molecular test could detect Pseudomonas syringae pv. syringae type in nearly 1 h, and this is the first time that Lamp molecular detection of Pseudomonas syringae pv. syringae particularly on stone fruits is described and introduced. The obtained data confirmed that LAMP is a fast, cheap, and high specific method for the rapid detection of Pseudomonas syringae pv. syringae to the comparison of PCR and culture.

中文翻译：

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环介导的等温扩增：快速诊断丁香假单胞菌光伏的分子技术。核果丁香

致病细菌对农业造成重大的经济损失。这种细菌的检测被认为是植物病理学家防止疾病传播的持续关注。丁香假单胞菌PV。丁香科是影响全世界核果产量和质量的最重要细菌病原体之一。生化测定法（例如LOPAT和GATTa）是检测这种病原体的常用方法。在King's B选择性培养基上进行血清学测试和培养也可用于分离该细菌。选择性培养基由特定且有效的成分组成，可抑制混合培养中某些微生物的生长，同时允许其他微生物生长。这些仅用于所选微生物的生长。King's B培养基可用作从食品，化妆品样品，植物等中进行非选择性分离培养和生产假单胞菌物种的色素的通用培养基。尽管如此，上述方法仍不足以区分菌株。另一方面，基于PCR的技术在检测植物病害方面既灵敏又有效。但是，这些技术对那些无法使用热循环仪的研究人员不可行。我们已经使用环介导的等温扩增来与靶标偶联。使用环引物和缓冲引物扩增syrD基因可用于预防疾病传播。这项研究的结果表明，与PCR相比，LAMP具有更高的敏感性。在LAMP和PCR副产物中，在微管中直接添加SYBR Gold比凝胶更敏感，因此我们可以消除凝胶电泳，而LAMP与通过培养获得的结果相比显示出高灵敏度和高特异性。所描述的分子测试可以检测丁香假单胞菌PV。丁香型近1小时，这是Lamp分子首次检测丁香假单胞菌pv。描述并介绍了丁香丁香科，特别是在核果上。获得的数据证实，LAMP是快速，廉价，高特异性的快速检测丁香假单胞菌PV的方法。丁香科的PCR和培养的比较。所描述的分子测试可以检测丁香假单胞菌PV。丁香型近1小时，这是Lamp分子首次检测丁香假单胞菌pv。描述并介绍了丁香丁香科，特别是在核果上。获得的数据证实，LAMP是快速，廉价，高特异性的快速检测丁香假单胞菌PV的方法。丁香科的PCR和培养的比较。所描述的分子测试可以检测到丁香假单胞菌。丁香型近1小时，这是Lamp分子首次检测丁香假单胞菌pv。描述并介绍了丁香丁香科，特别是在核果上。获得的数据证实，LAMP是快速检测丁香假单胞菌pv的快速，廉价和高特异性方法。丁香科的PCR和培养的比较。