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Improved Wound Closure Rates and Mechanical Properties Resembling Native Skin in Murine Diabetic Wounds Treated with a Tropoelastin and Collagen Wound Healing Device
bioRxiv - Bioengineering Pub Date : 2020-10-02 , DOI: 10.1101/2020.10.01.322636
Robert S. Kellar , Robert B. Diller , Aaron J. Tabor , Dominic D. Dominguez , Robert G. Audet , Tatum A. Bardsley , Alyssa J. Talbert , Nathan Cruz , Alison Ingraldi , Burt D. Ensley

Chronic wounds in patients suffering from type II diabetes mellitus (DMII) where wounds remain open with a complicated pathophysiology, healing, and recovery process is a public health concern. Normal wound healing plays a critical role in wound closure, restoration of mechanical properties, and the biochemical characteristics of the remodeled tissue. Biological scaffolds provide a tissue substitute to help facilitate wound healing by mimicking the extracellular matrix (ECM) of the dermis. In the current study an electrospun biomimetic scaffold, wound healing device (WHD), containing tropoelastin (TE) and collagen was synthesized to mimic the biochemical and mechanical characteristics of healthy human skin. The WHD was compared to a commercially available porcine small intestinal submucosa (SIS) matrix that has been used in both partial and full-thickness wounds, Oasis Wound Matrix. Wound closure rates, histochemistry, qPCR, and mechanical testing of treated wound sites were evaluated. The WHD in a splinted, full-thickness, diabetic murine wound healing model demonstrated an enhanced rate of wound closure, decreased tissue inflammation, skin organ regeneration, and a stronger and more durable remodeled tissue that more closely mimics native unwounded skin compared to the control device.

中文翻译:

用弹性蛋白和胶原伤口愈合装置治疗的鼠糖尿病伤口,伤口闭合率和机械性能类似于天然皮肤,改善了

患有II型糖尿病(DMII)的患者的慢性伤口(伤口仍保持开放状态,具有复杂的病理生理,愈合和恢复过程)是公共卫生关注的问题。正常的伤口愈合在伤口闭合,恢复机械特性以及重塑组织的生化特性方面起着至关重要的作用。生物支架通过模仿真皮的细胞外基质(ECM)提供组织替代物,以帮助促进伤口愈合。在当前的研究中,合成了包含原弹性蛋白(TE)和胶原蛋白的静电纺丝仿生支架,伤口愈合装置(WHD),以模仿人类健康皮肤的生物化学和机械特性。将WHD与已在部分和全层伤口中使用的市售猪小肠粘膜下层(SIS)基质Oasis伤口基质进行了比较。评估伤口闭合率,组织化学,qPCR和治疗伤口部位的机械测试。与对照相比,WHD在夹板的全厚度糖尿病鼠伤口愈合模型中显示出伤口闭合率提高,组织炎症减少,皮肤器官再生以及更坚固,更耐用的重塑组织,与原始未受伤的皮肤更加相似设备。
更新日期:2020-10-04
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