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A novel phage display vector for selection of target-specific peptides
Protein Engineering, Design and Selection ( IF 2.4 ) Pub Date : 2020-10-03 , DOI: 10.1093/protein/gzaa023
Alex Chang 1 , Joey P Ting 2 , Alfonso Espada 3 , Howard Broughton 3 , Manuel Molina-Martin 3 , Sepideh Afshar 2
Affiliation  

Intrinsic low display level of polypeptides on phage is a fundamental and limiting hurdle in successful isolation of target-specific binders by phage display technology. To circumvent this challenge, we optimized the copy number of peptides displayed on the phage surface using type 33 phage vector. We randomized the first 67 amino acids of the wild type PIII to identify mutants that would result in its reduced expression. Consequently, the display level was improved by 30-fold due to higher incorporation of the synthetic PIII–peptide fusion protein on the phage surface. Utilization of this novel phage vector should provide a solid basis for the discovery of therapeutic peptides.

中文翻译:

用于选择靶标特异性肽的新型噬菌体展示载体

噬菌体上多肽的固有低水平展示是通过噬菌体展示技术成功分离靶标特异性结合剂的基本和限制性障碍。为了规避这一挑战,我们使用33型噬菌体载体优化了在噬菌体表面展示的肽的拷贝数。我们随机化了野生型PIII的前67个氨基酸,以鉴定导致其表达降低的突变体。因此,由于在噬菌体表面上掺入了合成的PIII-肽融合蛋白,展示水平提高了30倍。这种新型噬菌体载体的使用应为发现治疗性肽提供坚实的基础。
更新日期:2020-10-04
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