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GhTULP34, a member of tubby-like proteins, interacts with GhSKP1A to negatively regulate plant osmotic stress
Genomics ( IF 4.4 ) Pub Date : 2020-10-03 , DOI: 10.1016/j.ygeno.2020.09.024
Zhanshuai Li 1 , Xiaoyan Wang 2 , Xiaocong Cao 3 , Baizhi Chen 3 , Changkai Ma 3 , Jiaoyan Lv 3 , Zhimao Sun 3 , Kaikai Qiao 3 , Longfu Zhu 4 , Chaojun Zhang 3 , Shuli Fan 1 , Qifeng Ma 3
Affiliation  

Tubby-like protein genes (TULPs), present in the form of large multigene families, play important roles in environmental stress. However, little is known regarding the TULP family genes in cotton. In this study, we systematically identified and analyzed the membership, characterization, and evolutionary relationship of TULPs in four species of cotton. Transcriptome analysis indicated that GhTULPs participate in environmental stress and cotton tissue development. At the same time, we also predicted and analyzed the potential molecular regulatory mechanisms and functions of TULPs. GhTULP34, as a candidate gene, significantly reduced the germination rate of transgenic Arabidopsis plants under salt stress, and inhibited root development and stomatal closure under mannitol stress. The yeast two-hybrid and luciferase (LUC) systems showed that GhTULP34 can interact with GhSKP1A, a subunit of the SCF-type (Skp1-Cullin-1-F-box) complex. This study will provide a basis and reference for future research on their roles in stress tolerance.



中文翻译:

GhTULP34,一个管状蛋白的成员,与 GhSKP1A 相互作用负调节植物渗透胁迫

Tubby 样蛋白基因 ( TULPs ) 以大型多基因家族的形式存在,在环境压力中发挥重要作用。然而,关于棉花中的TULP家族基因知之甚少。在这项研究中,我们系统地识别和分析了TULP在四种棉花中的成员资格、特征和进化关系。转录组分析表明GhTULP参与环境胁迫和棉花组织发育。同时,我们还对TULPs潜在的分子调控机制和功能进行了预测和分析。GhTULP34作为候选基因,显着降低转基因植物的发芽率拟南芥植物在盐胁迫下,甘露醇胁迫下抑制根发育和气孔闭合。酵母双杂交和荧光素酶 (LUC) 系统表明 GhTULP34 可以与 SCF 型 (Skp1-Cullin-1-F-box) 复合物的亚基 GhSKP1A 相互作用。本研究将为今后研究其在应激耐受中的作用提供依据和参考。

更新日期:2020-10-04
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