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Characterization of medical relevant anaerobic microorganisms by isothermal microcalorimetry
Anaerobe ( IF 2.3 ) Pub Date : 2020-10-02 , DOI: 10.1016/j.anaerobe.2020.102282
Stéphane Corvec 1 , Eva Seiler 2 , Lei Wang 3 , Mercedes Gonzalez Moreno 3 , Andrej Trampuz 3
Affiliation  

Detection of anaerobe bacteria by culture methods requires appropriate media, special growth conditions, additional detection techniques and it typically takes several days. Therefore, anaerobes are often missed in patient specimens under routine culture conditions. Microcalorimetry may provide a simple and accurate real-time method for faster and better detection of anaerobes. An isothermal calorimeter which detect minimal changes of temperature over time was used for the calorimetric experiments. In order to find optimal growth conditions, seven reference or clinical strains of medical relevant anaerobe bacteria were tested under different circumstances. First, the strains were tested with different growth media. After determining the optimal medium for each strain, the gas phase was modified by adding 3 mL or 4 mL medium, to evaluate growth under conditions with less oxygen. Cooked Meat Medium was best supporting growth of the tested strains, including Cutibacterium acnes, Fusobacterium nucleatum, Finegoldia magna, Parvimonas micra, Bacteroides fragilis and Actinomyces odontolyticus, followed by thioglycolate. The best medium to detect Clostridioides difficile was H-Medium. All tested strains showed better growth in 4 mL medium than in 3 mL. The detection time ranged between 10 and 72 h. Our results demonstrated that the sensitivity and the detection time of anaerobe bacteria can be improved by isothermal calorimetry with optimization of growth conditions. Therefore, calorimetric detection, a practical, quick and easy-to-do method, has the potential to replace current microbiological methods.



中文翻译:

等温微量热法表征医学相关厌氧微生物

通过培养方法检测厌氧菌需要合适的培养基、特殊的生长条件和额外的检测技术,通常需要几天时间。因此,在常规培养条件下,患者标本中经常会遗漏厌氧菌。微量热法可以提供一种简单而准确的实时方法,用于更快更好地检测厌氧菌。使用等温量热计检测温度随时间的最小变化用于量热实验。为了寻找最佳生长条件,在不同情况下对七种医学相关厌氧菌的参考或临床菌株进行了测试。首先,用不同的生长培养基测试菌株。确定每个菌株的最佳培养基后,通过添加 3 mL 或 4 mL 培养基对气相进行修改,在氧气较少的条件下评估生长。熟肉培养基最能支持测试菌株的生长,包括痤疮角质杆菌、具核梭杆菌Finegoldia magna微小单胞脆弱拟杆菌溶牙放线菌,其次是巯基乙酸盐。检测艰难梭菌的最佳培养基是 H-培养基。所有测试的菌株在 4 mL 培养基中的生长都比在 3 mL 中更好。检测时间介于 10 和 72 小时之间。我们的结果表明,通过优化生长条件,等温量热法可以提高厌氧菌的灵敏度和检测时间。因此,量热检测是一种实用、快速且易于操作的方法,有可能取代当前的微生物学方法。

更新日期:2020-10-12
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