The aim of this study was to assess the potential of bacteria Escherichia coli ATCC 35218, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923 and yeast Pichia membranifaciens ZIM 2417 to adhere to wooden surfaces such as poplar (Populus sp.), Norway spruce (Picea abies), European beech (Fagus sylvatica), beech coated with the commercial Belinka oil food contact and disinfectant P3-oxonia active 150, and investigate their survival on the beech wood surface under different relative humidities (RH; 65%, 75%, 85%, 98%) and temperatures (10 °C, 20 °C, 27 °C/37 °C). To extend the research goals, the scanning electron microscopy (SEM) analysis was also performed. The adhesion was determined by the number of colony-forming units per mm2 of sample (CFU/mm2). Results showed that all tested bacteria and yeast were able to adhere to the wooden surfaces, although differences were observed according to strains and type of wood. It was evident that number of adhered cells of S. aureus was lower on spruce (3.62 × 103 CFU/mm2) compared to poplar and beech (1.09 × 105 and 2.11 × 104 CFU/mm2, respectively). Furthermore, oil and disinfectant promoted the adhesion of P. aeruginosa (155.93 and 130.50%, respectively) on the beech surfaces, while they had a strong inhibitory effect on the other tested microorganisms E. coli (87.44 and 88.44%, respectively), S. aureus (91.24 and 96.80%, respectively) and P. membranifaciens (92.45 and 87.24%, respectively). These findings are consistent with SEM micrographs. The current data also indicated that relative humidity and temperature significantly affected the adhesion of tested bacteria and yeast. The highest degree of adhesion was observed at a relative humidity of 98% and temperature of 20 and 37 °C for bacteria, or 20 and 27 °C for yeast. Thus, the knowledge of how these microorganisms adhere to wooden surfaces and which factors affect this phenomenon proves to be of great importance in order to avoid their colonization.

中文翻译：

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影响细菌大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌和酵母毕赤酵母对木质表面粘附的因素

本研究的目的是评估细菌大肠杆菌 ATCC 35218、铜绿假单胞菌 ATCC 27853、金黄色葡萄球菌 ATCC 25923 和酵母毕赤酵母 ZIM 2417 粘附在杨树（Populus sp.）等木质表面上的潜力。 abies)、欧洲山毛榉 (Fagus sylvatica)、涂有商业 Belinka 油食品接触和消毒剂 P3-oxonia active 150 的山毛榉，并研究它们在不同相对湿度（RH；65%、75%、85 %、98%）和温度（10 °C、20 °C、27 °C/37 °C）。为了扩展研究目标，还进行了扫描电子显微镜 (SEM) 分析。粘附力由每平方毫米样品的菌落形成单位数 (CFU/mm2) 确定。结果表明，所有测试的细菌和酵母都能够粘附在木质表面上，尽管根据木材的菌株和类型观察到了差异。很明显，与杨树和山毛榉（分别为 1.09 × 105 和 2.11 × 104 CFU/mm2）相比，云杉上的金黄色葡萄球菌粘附细胞数量（3.62 × 103 CFU/mm2）较低。此外，油和消毒剂促进了铜绿假单胞菌（分别为 155.93 和 130.50%）在山毛榉表面的粘附，而它们对其他测试微生物大肠杆菌（分别为 87.44 和 88.44%）、S . aureus（分别为 91.24 和 96.80%）和膜假单胞菌（分别为 92.45 和 87.24%）。这些发现与 SEM 显微照片一致。目前的数据还表明，相对湿度和温度显着影响了测试细菌和酵母的附着力。在 98% 的相对湿度和 20 和 37 °C 的细菌温度或 20 和 27 °C 的酵母温度下观察到最高程度的粘附。因此，了解这些微生物如何粘附在木质表面上以及影响这种现象的因素被证明是非常重要的，以避免它们的定植。