This research project aimed to study the eliciting effect of different concentrations of methyl jasmonate (MeJA) (0.0, 25, 50, 75 µM L⁻¹) on the production of furanocoumarins (psoralen and bergapten) by harvested cells of batch cultures of Ruta graveolens L. grown in liquid Murashige and Skoog (MS) medium supplemented with 2.0 mg L⁻¹ 2,4-Dichlorophenoxy acetic acid (2,4-D) combined with 0.5 mg L⁻¹ Kinetin (Kin), during three incubation periods (7, 14 and 21 days). The highest concentration of Psoralen recorded was 3.220 mg g⁻¹ in the nutritional medium in a culture treated with 25 µM L⁻¹ MeJA after 21 days incubation. Bergapten reached its highest concentration of 8.223 mg g⁻¹ in the medium after 21 days incubation in a culture treated with 50 µM L⁻¹ MeJA. Harvested cells showed a different picture, Psoralen highest concentration (3.495 mg g⁻¹) was achieved after 14 days incubation in a culture treated with 75 µM L⁻¹ MeJA, while the highest concentration of bergapten (7.110 mg g⁻¹) was recorded after 21 days of incubation with 50 µM L⁻¹ MeJA. These data support the potential use of cell suspension cultures as an alternative source for the field plants in the production of furanocoumarins from Ruta graveolens.

该研究项目旨在研究不同浓度的茉莉酮酸甲酯（茉莉酸甲酯）（0.0，25，50，75μM升的引发效果^-1通过分批培养物的收获的细胞）对生产的呋喃香豆素（补骨脂素和佛手柑内酯）芸在三个孵育期中，在补充有2.0 mg L ^-1 2,4-二氯苯氧基乙酸（2,4-D）和0.5 mg L ^-1 Kinetin（Kin）的Murashige和Skoog（MS）液体培养基中生长的L. 7、14和21天）。孵育21天后，在用25 µM L ^-1 MeJA处理的培养物中的营养培养基中记录的补骨脂素的最高浓度为3.220 mg g ^-1。香柠檬素达到其最高浓度8.223 mg g^-1在21天后在培养用50μM大号处理的培养的培养基^-1茉莉酸甲酯。收获的细胞显示出不同的图像，在用75 µM L ^-1 MeJA处理的培养物中培养14天后，补骨脂素的最高浓度（3.495 mg g ^-1）达到最高，而记录的佛手柑最高浓度（7.110 mg g ^-1）与50 µM L ^-1 MeJA孵育21天后。这些数据支持了将细胞悬浮培养物作为田间植物从Rutagravolens生产呋喃香豆素的替代来源的潜在用途。