Plant responses to heavy metals and their storage constitute a crucial step to understand the environmental impacts of metallic trace elements (MTEs). In controlled experiments, we previously demonstrated the tolerance and resilience of Japanese knotweed to soil artificial polymetallic contamination. Using the same experimental design, we tested here the effect of three individual MTEs on Fallopia × bohemica performance traits. Rhizome fragments from three different sites (considered as distinct morphotypes) were grown in a greenhouse for 1 month on a prairial soil artificially contaminated with either Cd, Cr (VI) or Zn at concentrations corresponding to relatively highly polluted soils. Our results confirmed the high tolerance of Bohemian knotweed to metal stress, though, plant response to MTE pollution was dependant on MTE identity. Bohemian knotweed was stimulated by Cr (VI) (increased root and aerial masses), did not display any measurable change in performance traits under Cd at the high dose of 10 mg kg^-1, and uptook all MTEs in its rhizome, but only Zn was transferred to its aerial parts. We also highlighted changes in root secondary metabolism that were more accentuated with Zn, including the increase of anthraquinone, stilbene and biphenyl derivatives. These results compared to multi-contamination experiments previously published suggest complex interactions between metals and plant, depending principally on metal identity and also suggest a potential role of soil microbes in the interactions.

工厂对重金属及其储存的反应是了解金属微量元素（MTE）对环境的影响的关键步骤。在对照实验中，我们先前证明了虎杖对土壤人工多金属污染的耐受性和复原力。使用相同的实验设计，我们在这里测试了三个独立MTES的效果何首乌× bohemica性能特征。来自三个不同地点的根茎片段（被认为是不同的形态型）在温室中的人工土壤中生长了1个月，该土壤被Cd，Cr（VI）或Zn人工污染，其浓度与污染程度相对较高的土壤相对应。我们的结果证实了波希米亚虎杖对金属胁迫的高度耐受性，尽管植物对MTE污染的反应取决于M​​TE的特性。Cr（VI）（增加的根和气团）刺激波希米亚虎杖，在高剂量10 mg kg ^-1的Cd下，其性状未显示任何可测量的变化，并吸收了其根茎中的所有MTE，但只有Zn被转移到其空中部分。我们还着重指出了锌引起的根部次生代谢变化，其中包括蒽醌，二苯乙烯和联苯衍生物的增加。与先前发表的多污染实验相比，这些结果表明金属与植物之间的复杂相互作用（主要取决于金属特性），还表明了土壤微生物在相互作用中的潜在作用。