The fluorescent probe L, based on naphthalimide-modified coumarin, was designed, synthesized, and characterized, which could recognize Cu²⁺ from other cations selectively and sensitively in HEPES buffer (10 mM, Ph = 7. 4)/CH₃CN (1:4, V/V). When the probe L interacted with Cu²⁺, the color and the fluorescent intensity changed obviously and it provided the naked-eye detection for Cu²⁺. The recognition mode between them was achieved by Job's plot, IR, MS, SEM, and ¹HNMR. In addition, test strips made from L could still interact with Cu²⁺ in tap water effectively. The limit of detection (LOD) of L was 3.5 × 10⁻⁶ M. Additionally, the density functional theory (DFT) calculation method was used to analyze the action mechanism of L toward Cu²⁺. Importantly, the fluorescent probe L could demonstrate favorable selectivity toward Cu²⁺ in Caenorhabditis elegans. Thus, L was considered to have some potential for application in bioimaging.

荧光探针L基于萘酰亚胺修饰的香豆素，设计、合成并表征了它，它可以在 HEPES 缓冲液（10 mM，Ph = 7. 4）/CH ³ _CN（1:4， V/V）。当探头L与Cu ^{2+相互作用，颜色和荧光强度发生明显变化，为Cu 2+}的肉眼检测提供了依据。通过Job's图、IR、MS、SEM、^1HNMR等手段实现了它们之间的识别模式。此外，测试条由L仍能与自来水中的Cu ^{2+有效相互作用。}检测限 (LOD)L为3.5 × 10 ^-6 M。此外，采用密度泛函理论（DFT）计算方法分析了其作用机制L朝向Cu ²⁺。重要的是，荧光探针L可以表现出对 Cu 2+ 良好的^选择性秀丽隐杆线虫。因此，L被认为在生物成像方面具有一定的应用潜力。