Abstract

Sodium benzoate is a widely used food and pharmaceutical preservative due to its antibacterial and antifungal activity. In the present study effect of different concentrations of sodium benzoate on hepatic antioxidants, inflammatory cytokines (TNF-α, IFN-γ, IL-1β and IL-6), biochemical markers and histopathology of liver was evaluated. Twenty five adult rats (aged 1–2 months) with 5 rats per group were randomly distributed into 5 groups. Group 1 rats were used as control and all groups (1–5) were provided with water and fed ad libitum. In addition to usual water and food, rats of group 2, 3, 4 and 5 were treated with 70, 200, 400 and 700 mg/kg b.wt of sodium benzoate once a day via oral gavage for 30 days. Our results showed that activity of glutathione peroxidase (GPx), catalase (CAT), glutathione-s-transferase (GST), glutathione reductase (GR) and superoxide dismutase (SOD) in rats decreased significantly when treated with 200, 400 and 700 mg/kg b.wt of sodium benzoate. Increase in the concentration of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, serum total protein, albumin, globulin, urea and creatinine was found to be dose dependent. Severe histopathological damage was observed in the hepatic tissue at higher concentrations of sodium benzoate. It was noticed that high concentrations of sodium benzoate (200, 400 and 700 mg/kg b.wt) produce significant increase in inflammatory cytokine markers (TNF-α, IFN-γ, IL-1β and IL-6) in comparison to control. Sodium benzoate at concentration of 70 mg/kg b.wt did not produce any significant changes in any of the above studied parameters.

摘要

苯甲酸钠是一种广泛使用的食品和药品防腐剂，因为它具有抗菌和抗真菌活性。本研究评估了不同浓度苯甲酸钠对肝脏抗氧化剂、炎性细胞因子（TNF-α、IFN-γ、IL-1β和IL-6）、生化标志物和肝脏组织病理学的影响。将 25 只成年大鼠（1～2 月龄），每组 5 只，随机分为 5 组。第 1 组大鼠用作对照组，所有组 (1-5) 均提供水并随意喂食。除了通常的水和食物外，第 2、3、4 和 5 组的大鼠每天一次通过70、200、400 和 700 mg/kg b.wt 苯甲酸钠处理灌胃30天。我们的研究结果表明，当用 200、400 和 700 mg 治疗时，大鼠中谷胱甘肽过氧化物酶 (GPx)、过氧化氢酶 (CAT)、谷胱甘肽-s-转移酶 (GST)、谷胱甘肽还原酶 (GR) 和超氧化物歧化酶 (SOD) 的活性显着降低/kg b.wt苯甲酸钠。发现丙氨酸氨基转移酶、天冬氨酸氨基转移酶、碱性磷酸酶、血清总蛋白、白蛋白、球蛋白、尿素和肌酐浓度的增加呈剂量依赖性。在较高浓度的苯甲酸钠下，在肝组织中观察到严重的组织病理学损伤。值得注意的是，与对照相比，高浓度苯甲酸钠（200、400 和 700 mg/kg b.wt）会显着增加炎症细胞因子标志物（TNF-α、IFN-γ、IL-1β 和 IL-6） .