Here it is reported on the fabrication of a carbon‐gold micro/nano hierarchical platform using a stimuli‐responsive wrinkling technology. The approach exploits the generation of depth wise photo‐crosslinking gradient in SU8 thin films doped with a UV light absorbing gold precursor. Wrinkled films are then pyrolyzed at 900 °C, resulting in well preserved carbonized wrinkle patterns along with the growth of GNPs. The wrinkling phenomenon is explained using a theoretical model that includes the influence of the film thickness, the gold precursor concentration, and the exposure gradient that sets the theoretical intermolecular distance of free monomers along the film's depth direction. The predicted evolution of the wrinkle patterns is supported by experimental results. Surface roughness and wetting properties of the resulting topographies are studied in detail. GNPs act as potential anchor points for cell adhesion whereas mechanical topographies controls the cellular alignment as a result of contact guidance. Fibronectin functionalization significantly improves cell attachment, biocompatibility, and provides highly aligned cell cultures. This whole strategy of fabricating non‐planar carbon/gold‐based cell culture platform has great potential for their use in lab on a chip and tissue engineering based applications.

中文翻译：

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碳纳米金分层微/纳米地形图指导细胞

此处报道了使用刺激响应性起皱技术制造碳金微/纳米分级平台的过程。该方法利用在掺有吸收紫外线的金前体的SU8薄膜中生成深度光交联梯度。然后将起皱的薄膜在900°C的温度下热解，导致保存良好的碳化皱纹图案以及GNP的生长。使用理论模型来解释起皱现象，该理论模型包括膜厚度，金前驱体浓度和曝光梯度的影响，该梯度设置了沿膜的深度方向的自由单体的理论分子间距离。实验结果支持了皱纹模式的预测演变。详细研究了所得表面形貌的表面粗糙度和润湿性能。GNPs作为细胞粘附的潜在锚点，而机械形貌由于接触引导而控制细胞排列。纤连蛋白功能化可显着改善细胞附着，生物相容性并提供高度对齐的细胞培养物。制造基于非平面碳/金的细胞培养平台的整个策略具有很大的潜力，可用于基于芯片和组织工程的实验室应用。