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Draft genome sequence of plastic degrading Bacillus sp. AIIW2 isolated from the Arabian ocean
Journal of Basic Microbiology ( IF 3.1 ) Pub Date : 2020-10-01 , DOI: 10.1002/jobm.202000416
Alka Kumari 1 , Nasreen Bano 2, 3 , Doongar R Chaudhary 1, 3 , Bhavanath Jha 1
Affiliation  

The endemic spread of plastic in the environment requires urgent need of a sustainable approach. Marine microbes found to have vast bioactivity and play a central role in biogeochemical cycling in the ocean; however, very few of them had been explored for biochemical cycling or plastic degradation. In the present study, we report the draft genome sequence of marine Bacillus sp. AIIW2 which was found to utilize plastic as a carbon source. The Bacillus sonorensis SRCM101395 was used as a reference genome for mapping the reads. The genome size of strain AIIW2 was approximately 4.4 Mb and composed of 4737 coding sequences with 45.7% G + C contents. The whole genome comparison of strain AIIW2 with three closest Bacillus strains showed strain specificity, the 16S ribosomal RNA sequence shows 99.93% similarity with Bacillus paralicheniformis KJ‐16T (KY694465). This genome data would provide the genetic basis in developing plastic bioremediation approaches and discover the enzymes pertinent in the biodegradation processes.

中文翻译:

塑料降解芽孢杆菌的基因组序列草图。AIIW2 从阿拉伯洋中分离出来

塑料在环境中的地方性传播迫切需要一种可持续的方法。发现海洋微生物具有巨大的生物活性,并在海洋生物地球化学循环中发挥核心作用;然而,很少有人对其中的生物化学循环或塑料降解进行过探索。在本研究中,我们报告了海洋芽孢杆菌的基因组序列草图。AIIW2 被发现利用塑料作为碳源。声纳芽孢杆菌 SRCM101395 用作绘制读数的参考基因组。菌株 AIIW2 的基因组大小约为 4.4 Mb,由 4737 个编码序列组成,G + C 含量为 45.7%。菌株AIIW2与三个最接近的芽孢杆菌菌株的全基因组比较显示菌株特异性,16S核糖体RNA序列显示99。与 Bacillus paralicheniformis KJ-16T (KY694465) 具有 93% 的相似性。该基因组数据将为开发塑料生物修复方法提供遗传基础,并发现与生物降解过程相关的酶。
更新日期:2020-10-01
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