Isolated myofibers are commonly used to understand the function of skeletal muscle in vivo. This can involve single isolated myofibers obtained from dissection or from enzymatic dissociation. Isolation via dissection allows control of sarcomere length and preserves tendon attachment but is labor-intensive, time-consuming and yields few viable myofibers. In contrast, enzymatic dissociation is fast and facile, produces hundreds of myofibers, and more importantly reduces the number of muscles/animals needed for studies. Biomechanical properties of the sarcolemma have been studied using myofibers from the extensor digitorum longus, but this has been limited to dissected myofibers, making data collection slow and difficult. We have modified this tool to perform biomechanical measurements of the sarcolemma in dissociated myofibers from the flexor digitorum brevis.

中文翻译：

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肌纤维分离技术对肌膜生物力学的影响。

分离的肌纤维通常用于了解体内骨骼肌的功能. 这可能涉及从解剖或酶解中获得的单个分离的肌纤维。通过解剖隔离可以控制肌节长度并保留肌腱附着，但劳动密集型、耗时且几乎不能产生可行的肌纤维。相比之下，酶解快速而容易，产生数百个肌纤维，更重要的是减少了研究所需的肌肉/动物的数量。已经使用来自趾长伸肌的肌纤维研究了肌膜的生物力学特性，但这仅限于解剖的肌纤维，这使得数据收集缓慢且困难。我们已修改此工具，以对来自趾短屈肌的分离肌纤维中的肌膜进行生物力学测量。