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Optimizing Medium Components to Enhance High Cell Mass Production of Biotherapeutic Strain Lactobacillus reuteri DSM 20016T by Statistical Method
Journal of Scientific & Industrial Research ( IF 0.6 ) Pub Date : 2020-10-01
S Selvamani, D J Dailin, M Rostom, R A Malek, V K Gupta, H A El-Enshasy

Probiotics referred to a group of living microorganisms which highly influence the human health. A number of studies have highlighted on the bio-therapeutic potential of Lactobacillus reuteri strains, especially in treating eradication of Helicobacter pylori. Therefore, in present investigation, statistical methods were applied to optimize medium composition for high cell mass production of L. reuteri strain DSM 20016T. Most influencing medium components were screened by using Plackett-Burman and optimized using Box-Bhenken experimental design. The concentration of lactose, yeast extract and phosphate in cultivation medium has shown significant effect on the cell mass production. The highest cell mass obtained after 48h incubation was 3.96 ± 0.02 gL−1 in RSM-optimized medium compared to 1.76 ± 0.17 gL−1 in un-optimized medium.

中文翻译:

通过统计方法优化培养基成分以增强生物治疗菌株路透乳杆菌DSM 20016T的大细胞量生产

益生菌是指对人体健康有高度影响的一组活微生物。大量研究强调了路氏乳杆菌菌株的生物治疗潜力,特别是在根除幽门螺杆菌方面。因此,在本研究中,采用统计学方法来优化培养基的组成,以大量生产罗伊氏乳杆菌DSM 20016 T。使用Plackett-Burman筛选了大多数影响培养基的成分,并使用Box-Bhenken实验设计进行了优化。培养基中乳糖,酵母提取物和磷酸盐的浓度已显示出对细胞大量生产的显着影响。孵育48h后获得的最高细胞量在RSM优化培养基中为3.96±0.02 gL -1,而在未优化培养基中为1.76±0.17 gL -1
更新日期:2020-10-02
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