Monitoring the mutation and evolution of the virus is important for tracing its ongoing transmission and facilitating effective vaccine development. A total of 342 complete genomic sequences of SARS-CoV-2 were analyzed in this study. Compared to the reference genome reported in December 2019, 465 mutations were found, among which, 347 occurred in only 1 sequence, while 26 occurred in more than 5 sequences. For these 26 further identified as SNPs, 14 were closely linked and were grouped into 5 profiles. Phylogenetic analysis revealed the sequences formed 2 major groups. Most of the sequences in late period (March and April) constituted the Cluster II, while the sequences before March in this study and the reported S/L and A/B/C types in previous studies were all in Cluster I. The distributions of some mutations were specific geographically or temporally, the potential effect of which on the transmission and pathogenicity of SARS-CoV-2 deserves further evaluation and monitoring. Two mutations were found in the receptor-binding domain (RBD) but outside the receptor-binding motif (RBM), indicating that mutations may only have marginal biological effects but merit further attention. The observed novel sequence divergence is of great significance to the study of the transmission, pathogenicity, and development of an effective vaccine for SARS-CoV-2.

监测病毒的突变和进化对于追踪其持续传播和促进有效的疫苗开发非常重要。本研究共分析了 342 条 SARS-CoV-2 的完整基因组序列。与2019年12月报告的参考基因组相比，共发现465个突变，其中347个仅发生在1个序列中，26个发生在5个以上序列中。对于这 26 个进一步确定为 SNP 的 SNP，其中 14 个密切相关并被分为 5 个配置文件。系统发育分析显示这些序列形成了 2 个主要组。后期（3月和4月）序列大部分构成Cluster II，而本研究3月之前的序列以及之前研究报道的S / L和A / B / C类型都在Cluster I中。一些突变的分布在地理或时间上是特定的，其对 SARS-CoV-2 的传播和致病性的潜在影响值得进一步评估和监测。在受体结合域 (RBD) 但在受体结合基序 (RBM) 之外发现了两个突变，表明突变可能仅具有边缘的生物学效应，但值得进一步关注。观察到的新序列差异对于研究 SARS-CoV-2 的传播、致病性和开发有效疫苗具有重要意义。表明突变可能仅具有边际生物学效应，但值得进一步关注。观察到的新序列差异对于研究 SARS-CoV-2 的传播、致病性和开发有效疫苗具有重要意义。表明突变可能仅具有边际生物学效应，但值得进一步关注。观察到的新序列差异对于研究 SARS-CoV-2 的传播、致病性和开发有效疫苗具有重要意义。