The banana aphid, Pentalonia nigronervosa Coquerel (Hemiptera: Aphididae), is a major pest of cultivated bananas (Musa spp., order Zingiberales), primarily due to its role as a vector of Banana bunchy top virus (BBTV), the most severe viral disease of banana worldwide. Here, we generated a highly complete genome assembly of P. nigronervosa using a single PCR-free Illumina sequencing library. Using the same sequence data, we also generated complete genome assemblies of the P. nigronervosa symbiotic bacteria Buchnera aphidicola and Wolbachia. To improve our initial assembly of P. nigronervosa we developed a k-mer based deduplication pipeline to remove genomic scaffolds derived from the assembly of haplotigs (allelic variants assembled as separate scaffolds). To demonstrate the usefulness of this pipeline, we applied it to the recently generated assembly of the aphid Myzus cerasi, reducing the duplication of conserved BUSCO genes by 25%. Phylogenomic analysis of P. nigronervosa, our improved M. cerasi assembly, and seven previously published aphid genomes, spanning three aphid tribes and two subfamilies, reveals that P. nigronervosa falls within the tribe Macrosiphini, but is an outgroup to other Macrosiphini sequenced so far. As such, the genomic resources reported here will be useful for understanding both the evolution of Macrosphini and for the study of P. nigronervosa. Furthermore, our approach using low cost, high-quality, Illumina short-reads to generate complete genome assemblies of understudied aphid species will help to fill in genomic black spots in the diverse aphid tree of life.

香蕉蚜虫，Pentalonia nigronervosa Coquerel（半翅目：蚜科），是种植香蕉的主要害虫（芭蕉属，为了姜类），主要是由于其作为一个载体的作用香蕉束顶病毒（BBTV） ，最严重的病毒世界范围内的香蕉病。在这里，我们使用单个无PCR的Illumina测序文库生成了黑 腹果蝇的高度完整的基因组组装。使用相同的序列数据，我们也产生了全基因组组装P. nigronervos共生细菌鬼羽箭属aphidicola和沃尔巴克氏体。为了改善我们对P的初始组装。 nigronervos a我们开发了一种基于k-mer的重复数据删除管道，以去除源自单倍体组装（等位基因变体组装为单独的支架）的基因组支架。为了证明此管道的有用性，我们将其应用于最近产生的蚜虫Myzus cerasi的装配，将保守BUSCO基因的重复减少了25％。的Phylogenomic分析P. nigronervos一个，我们的改进M.樱桃黑色轮斑组装，七个先前公布的蚜虫基因组，跨越了三个蚜虫部落和两个亚科，表明P. nigronervos一个属于Macrosiphini部落，但与迄今排序的其他Macrosiphini相比属于一个群体。因此，基因组资源的报道，这里将成为了解Macrosphini双方的发展和研究的有用P. nigronervos一个。此外，我们使用低成本，高质量，Illumina短读本来生成未充分研究的蚜虫物种的完整基因组组装体的方法，将有助于填补各种蚜虫生命树中的基因组黑点。